过氧化物酶体增殖物激活受体 γ 调控乳脂合成及乳脂分泌相关基因在奶山羊乳腺上皮细胞中的表达。

PPAR γ Regulates Genes Involved in Triacylglycerol Synthesis and Secretion in Mammary Gland Epithelial Cells of Dairy Goats.

机构信息

Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.

出版信息

PPAR Res. 2013;2013:310948. doi: 10.1155/2013/310948. Epub 2013 Apr 17.

To explore the function of PPAR γ in the goat mammary gland, we cloned the whole cDNA of the PPAR γ gene. Homology alignments revealed that the goat PPAR γ gene is conserved among goat, bovine, mouse, and human. Luciferase assays revealed that rosiglitazone enhanced the activity of the PPAR γ response element (PPRE) in goat mammary epithelial cells (GMECs). After rosiglitazone (ROSI) treatment of GMECs, there was a significant (P < 0.05) increase in the expression of genes related to triacylglycerol synthesis and secretion: LPL, FASN, ACACA, PLIN3, FABP3, PLIN2, PNPLA2, NR1H3, SREBF1, and SCD. The decreases in expression observed after knockdown of PPAR γ relative to the control group (Ad-NC) averaged 65%, 52%, 67%, 55%, 65%, 58%, 85%, 43%, 50%, and 24% for SCD, DGAT1, AGPAT6, SREBF1, ACACA, FASN, FABP3, SCAP, ATGL, and PLIN3, respectively. These results provide direct evidence that PPAR γ plays a crucial role in regulating the triacylglycerol synthesis and secretion in goat mammary cells and underscore the functional importance of PPAR γ in mammary gland tissue during lactation.

为了探索 PPARγ 在山羊乳腺中的功能，我们克隆了 PPARγ 基因的全长 cDNA。同源性比对表明，山羊 PPARγ 基因在山羊、牛、小鼠和人类中是保守的。荧光素酶报告基因检测显示，罗格列酮增强了山羊乳腺上皮细胞（GMECs）中 PPARγ 反应元件（PPRE）的活性。在 GMECs 用罗格列酮（ROSI）处理后，与对照组（Ad-NC）相比，与三酰基甘油合成和分泌相关的基因表达显著增加：LPL、FASN、ACACA、PLIN3、FABP3、PLIN2、PNPLA2、NR1H3、SREBF1 和 SCD。与对照组（Ad-NC）相比，PPARγ 敲低后观察到的基因表达平均下降了 65%、52%、67%、55%、65%、58%、85%、43%、50%和 24%，分别为 SCD、DGAT1、AGPAT6、SREBF1、ACACA、FASN、FABP3、SCAP、ATGL 和 PLIN3。这些结果为 PPARγ 在山羊乳腺细胞中调节三酰基甘油合成和分泌中发挥关键作用提供了直接证据，并强调了 PPARγ 在泌乳期乳腺组织中的功能重要性。