Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
Biomed Res Int. 2013;2013:795103. doi: 10.1155/2013/795103. Epub 2012 Dec 31.
The aim of the study was to investigate the association between elevated serum fetuin-A and increased urine albumin excretion in obese rats, and whether increased urine albumin excretion was modified by improving hepatic steatosis and lipid metabolism disorder. Male Wistar rats 4 weeks in age were randomly divided into three groups and fed with normal chow (control group), high-fat chow (obesity group), or high-fat chow plus fenofibrate (fenofibrate group). After 24 weeks, both body weight and visceral fat/body weight ratio in obese rats were higher than in controls. A difference in serology markers and pathology associated with hepatic steatosis was also found among the three groups. Serum fetuin-A and the expression of NF- κ B in the liver were increased, while serum adiponectin was decreased in obese rats in comparison to controls (P < 0.01). Urinary albumin/creatinine ratio (ACR) was increased in the obesity group compared to controls (P < 0.01). The fenofibrate intervention reduced serum fetuin-A and NF- κ B expression in the liver and increased serum adiponectin compared to obese rats and was accompanied by decrease in ACR. A positive correlation was found between ACR and fetuin-A (r = 0.602, P < 0.01), and a negative correlation was found between ACR and adiponectin (r = -0.635, P < 0.01). We conclude that elevated fetuin-A levels are associated with microalbuminuria in obese rats, and abnormal albuminuria is reversible by improving hepatic steatosis.
本研究旨在探讨肥胖大鼠血清胎球蛋白 A 升高与尿白蛋白排泄增加之间的关系，以及改善肝脂肪变性和脂质代谢紊乱是否会改变尿白蛋白排泄。4 周龄雄性 Wistar 大鼠随机分为三组，分别给予普通饲料（对照组）、高脂饲料（肥胖组）或高脂饲料加非诺贝特（非诺贝特组）。24 周后，肥胖大鼠的体重和内脏脂肪/体重比均高于对照组。三组大鼠血清学标志物和与肝脂肪变性相关的病理学也存在差异。与对照组相比，肥胖大鼠血清胎球蛋白 A 和肝脏 NF- κ B 表达增加，而血清脂联素减少（P<0.01）。与对照组相比，肥胖组大鼠尿白蛋白/肌酐比值（ACR）升高（P<0.01）。与肥胖大鼠相比，非诺贝特干预降低了血清胎球蛋白 A 和肝脏 NF- κ B 的表达，增加了血清脂联素，并伴有 ACR 的降低。ACR 与胎球蛋白 A 呈正相关（r=0.602，P<0.01），与脂联素呈负相关（r=-0.635，P<0.01）。综上所述，肥胖大鼠血清胎球蛋白 A 水平升高与微量白蛋白尿有关，改善肝脂肪变性可使异常白蛋白尿逆转。
