Tang Y, Diao Y, Chen H, Ou Q, Liu X, Gao X, Yu C, Wang L
Institute of Poultry Disease, Shan Dong Agricultural University, Taian, Shan Dong Province, China.
Transbound Emerg Dis. 2015 Apr;62(2):209-16. doi: 10.1111/tbed.12111. Epub 2013 May 27.
Tembusu virus (TMUV) is a flavivirus, presumed to be a mosquito-borne flavivirus of the Ntaya virus subgroup. To date, however, there have been no reports indicating that mosquitoes are involved in the spread of TMUV. In this study, we report the first isolation of TMUV from Culex mosquitoes. We describe the isolation and characterization of a field strain of TMUV from mosquitoes collected in Shandong Province, China. The virus isolate, named TMUV-SDMS, grows well in mosquito cell line C6/36, in Vero and duck embryo fibroblast (DEF) cell lines, and causes significant cytopathic effects in these cell cultures. The TMUV-SDMS genome is a single-stranded RNA, 10 989 nt in length, consisting of a single open reading frame encoding a polyprotein of 3410 amino acids, with 5' and 3' untranslated regions of 142 and 617 nt, respectively. Phylogenetic analysis of the E and NS5 genes revealed that the TMUV-SDMS is closely related to the TMUV YY5 and BYD strains which cause severe egg-drop in ducks. The 3'NTR of TMUV-SDMS contains two pairs of tandem repeat CS and one non-duplicate CS, which have sequence similarities to the same repeats in the YY5 and BYD strains. Our findings indicate that mosquitoes carrying the TMUV may play an important role in the spread of this virus and in disease outbreak.
坦布苏病毒（TMUV）是一种黄病毒，被认为是恩塔亚病毒亚组中一种由蚊子传播的黄病毒。然而，迄今为止，尚无报告表明蚊子参与了TMUV的传播。在本研究中，我们报告了首次从库蚊中分离出TMUV。我们描述了从中国山东省采集的蚊子中分离出的一株TMUV野外毒株的特性。该病毒分离株名为TMUV-SDMS，在蚊子细胞系C6/36、非洲绿猴肾细胞系（Vero）和鸭胚成纤维细胞（DEF）系中生长良好，并在这些细胞培养物中引起明显的细胞病变效应。TMUV-SDMS基因组是一条单链RNA，长度为10989个核苷酸，由一个单一的开放阅读框组成，编码一个含有3410个氨基酸的多聚蛋白，5'和3'非翻译区分别为142和617个核苷酸。对E基因和NS5基因的系统发育分析表明，TMUV-SDMS与导致鸭严重产蛋下降的TMUV YY5和比亚迪毒株密切相关。TMUV-SDMS的3'非翻译区包含两对串联重复序列CS和一个非重复的CS，它们与YY5和比亚迪毒株中的相同重复序列具有序列相似性。我们的研究结果表明，携带TMUV的蚊子可能在该病毒的传播和疾病暴发中起重要作用。
