Center for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, Taipei City 11561, Taiwan.
Viruses. 2020 May 22;12(5):567. doi: 10.3390/v12050567.
We identified and isolated a novel Tembusu virus (TMUV) strain TP1906 (TMUV-TP1906) from a mosquito pool collected from the northern part of Taiwan in 2019. The TMUV-TP1906 genome is a 10,990-nucleotide-long, positive-sense, single-stranded RNA, consisting of a single open reading frame (ORF) encoding a polyprotein of 3425 amino acids, with 5' and 3' untranslated regions (UTRs) of 94 and 618 nucleotides, respectively. The nucleotide sequence of the TMUV-TP1906 of ORF exhibited 93.71% and 91.27% similarity with Sitiawan virus (STWV) and the TMUV prototype strain MM1775, respectively. The 3'-UTR variable region of TMUV-TP1906 showed nucleotide sequence divergence with other TMUV strains. Phylogenetic analysis of the complete ORF and polyprotein sequences revealed that TMUV-TP1906 is most closely related to STWV which causes encephalitis and retarded growth in chickens. We found that the TMUV-TP1906 caused a cytopathic effect (CPE) in the DF-1 chicken fibroblast cell line, while no apparent CPE was observed in Vero and C6/36 cells. In this study, we first identified and isolated a novel TMUV strain in Taiwan. In addition, to our knowledge, it is the first time that the TMUV strain was isolated from the mosquitoes. Further study is warranted to investigate the host range and virulence of TMUV-TP1906.
我们从 2019 年在台湾北部采集的蚊子池中鉴定并分离出一种新型的坦布苏病毒（TMUV）株 TP1906（TMUV-TP1906）。TMUV-TP1906 基因组是一条长 10990 个核苷酸的正链单股 RNA，由一个编码 3425 个氨基酸的多蛋白的单一开放阅读框（ORF）组成，其 5'和 3'非翻译区（UTR）分别为 94 和 618 个核苷酸。TMUV-TP1906 的 ORF 核苷酸序列与 Sitiawan 病毒（STWV）和 TMUV 原型株 MM1775 的相似度分别为 93.71%和 91.27%。TMUV-TP1906 的 3'-UTR 可变区与其他 TMUV 株的核苷酸序列存在差异。完整 ORF 和多蛋白序列的系统发育分析表明，TMUV-TP1906与引起鸡脑炎和生长迟缓的 STWV 最为密切相关。我们发现 TMUV-TP1906 可引起 DF-1 鸡成纤维细胞系的细胞病变效应（CPE），而在 Vero 和 C6/36 细胞中未观察到明显的 CPE。在这项研究中，我们首次在台湾鉴定并分离出一种新型的 TMUV 株。此外，据我们所知，这是首次从蚊子中分离出 TMUV 株。需要进一步研究以调查 TMUV-TP1906 的宿主范围和毒力。
