Karantanos Theodoros, Theodoropoulos George, Gazouli Maria, Vaiopoulou Anna, Karantanou Christina, Lymberi Maria, Pektasides Dimitrios
First Department of Propaedeutic Surgery, Athens Medical School, Athens - Greece.
Int J Biol Markers. 2013 Sep 27;28(3):280-5. doi: 10.5301/jbm.5000033.
Recent studies have demonstrated the influence of clock genes in cell cycle regulation, cell proliferation, apoptosis and DNA damage recognition and repair. There is evidence suggesting the implication of clock genes in colorectal cancer (CRC) development and progression. The aim of this study is to evaluate the expression levels of clock genes in CRC and correlate them with patients' prognosis. Forty-two CRC samples (from 24 males and 18 females), their paired noncancerous tissues and 8 biopsies from healthy individuals were included. Quantitative real-time PCR was used to examine the expression levels of CLOCK1, BMAL1, PER1, PER2 and PER3 genes in all the samples. In the cancerous tissues CLOCK1 (p<0.0001) and BMAL1 (p<0.0001) expression levels were higher, while PER1 (p<0.0024) and PER3 (p<0.0001) expression levels were lower compared to matched healthy tissues. No difference was observed in the expression levels of PER2 (p=0.99). No correlation was found between clock gene expression and patients' clinicopathological characteristics or prognosis. The results suggest abnormal expression of CLOCK1, BMAL1, PER1 and PER3 genes in CRC but no correlation with patients' prognosis.
近期研究已证实生物钟基因在细胞周期调控、细胞增殖、细胞凋亡以及DNA损伤识别与修复过程中的影响。有证据表明生物钟基因与结直肠癌（CRC）的发生发展有关。本研究旨在评估生物钟基因在CRC中的表达水平，并将其与患者的预后相关联。研究纳入了42例CRC样本（24例男性和18例女性）、其配对的癌旁组织以及8例健康个体的活检组织。采用定量实时PCR检测所有样本中CLOCK1、BMAL1、PER1、PER2和PER3基因的表达水平。与匹配的健康组织相比，癌组织中CLOCK1（p<0.0001）和BMAL1（p<0.0001）的表达水平较高，而PER1（p<0.0024）和PER3（p<0.0001）的表达水平较低。PER2的表达水平未观察到差异（p=0.99）。未发现生物钟基因表达与患者的临床病理特征或预后之间存在相关性。结果表明CRC中CLOCK1、BMAL1、PER1和PER3基因表达异常，但与患者预后无关。
