Department of Biotechnology, Panjab University, Chandigarh, India.
Mol Biol Rep. 2013 Aug;40(8):4921-8. doi: 10.1007/s11033-013-2592-5. Epub 2013 May 28.
The epigenetic modifications have been reported to be key factors in breast carcinogenesis. In the current study, it has been tried to determine the methylation status of two tumour suppressor genes p14/ARF and p16/INK4a in 150 breast cancer patients as well as 150 controls by using MSP-PCR. There was, highly significant difference in methylation of p14/ARF and p16/INK4a (P=0.000) between patients and controls. Methylation of both the genes together significantly increased the risk of breast cancer by 12.31 folds. The present study concludes that hypermethylation of p14/ARF and p16/INK4a promoters demonstrate significant association with the risk of breast cancer, hence indicating these as important tumour suppressor genes involved in the pathogenesis of breast cancer in North Indian population (i.e. Punjab, Haryana, Uttar Pradesh, Himachal Pradesh as well as Union Territory of Chandigarh).
表观遗传修饰已被报道为乳腺癌发生的关键因素。在本研究中，我们试图通过 MSP-PCR 检测 150 例乳腺癌患者和 150 例对照中两个肿瘤抑制基因 p14/ARF 和 p16/INK4a 的甲基化状态。患者和对照组之间 p14/ARF 和 p16/INK4a 的甲基化存在显著差异（P=0.000）。两个基因的甲基化共同使乳腺癌的发病风险增加了 12.31 倍。本研究的结论是，p14/ARF 和 p16/INK4a 启动子的高甲基化与乳腺癌的发病风险显著相关，表明它们是参与印度北部人群（即旁遮普邦、哈里亚纳邦、北方邦、喜马偕尔邦以及昌迪加尔联邦属地）乳腺癌发病机制的重要肿瘤抑制基因。
