Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB21QW, United Kingdom.
Biochemistry. 2013 Jun 25;52(25):4433-8. doi: 10.1021/bi400315s. Epub 2013 Jun 13.
The Pseudomonas aeruginosa quinolone signal (PQS) is a quorum sensing molecule that plays an important role in regulating the virulence of this organism. We have purified the ligand binding domain of the receptor PqsRLBD for PQS and have used Förster resonance energy transfer fluorimetry and kinetic modeling to characterize the ligand binding in vitro. The dissociation constant for binding of PQS to a ligand binding site in (PqsRLBD)2 dimers was determined to be 1.2 ± 0.3 μM. We found no cooperativity in the consecutive binding of two ligand molecules to the dimer.
铜绿假单胞菌群体感应信号（PQS）是一种群体感应分子，在调节该生物体的毒力方面发挥着重要作用。我们已经纯化了 PQS 的受体 PqsRLBD 的配体结合结构域，并使用荧光各向异性和动力学建模来表征体外的配体结合。确定 PQS 与（PqsRLBD）2 二聚体中的配体结合位点结合的解离常数为 1.2±0.3 μM。我们发现两个配体分子连续结合到二聚体上没有协同作用。
