The University of Agriculture , Peshawar, Khyber Pakhtunkhwa 25120, Pakistan.
J Agric Food Chem. 2013 Jun 19;61(24):5689-95. doi: 10.1021/jf401309r. Epub 2013 Jun 7.
Two bioactive nematicidal phytochemicals, viz., nonacosane-10-ol and 23a-homostigmast-5-en-3β-ol, were isolated from the n-hexane fraction of the roots of Fumaria parviflora through activity-guided isolation. The structures of the compounds were elucidated using ¹³C and ¹H nuclear magnetic resonance. Activity of the two compounds against eggs and juveniles (J2s) of Meloidogyne incognita was evaluated in vitro at the concentrations of 50, 100, 150, and 200 μg mL⁻¹. Over 120 h of incubation, the cumulative percent mortality and hatch inhibition of both of the compounds tested ranged from 20 to 100% and from 15 to 95.0%, respectively. In pot trials with tomato cultivar Riogrande, the two compounds, applied as soil drenches at the concentrations of 100, 200, and 300 mg/kg, significantly decreased the nematodes and plant growth parameters. Nonacosane-10-ol and 23a-homostigmast-5-en-3β-ol reduced the numbers of galls (42.6 and 60.3), galling index (1.6 and 2.8), females per gram of root (37.3 and 57.0), eggs per gram of root (991.3 and 1273.0), reproduction factor (Rf) (0.1 and 0.2), and fresh root weight (14.33 and 17.0 g) at 300 mg/kg concentration and increased fresh shoot weight (49.0 and 48.4 g), dry shoot weight (28.0 and 25.3 g), and plant height (53.5 and 49.6 cm), respectively. These compounds could provide new insight in the search for novel nematicides against M. incognita.
从紫堇属植物根的正己烷部分通过活性导向分离，分离出两种具有生物活性的杀线虫植物化学物质，即正二十九烷-10-醇和 23a-同型麦角甾-5-烯-3β-醇。使用 ¹³C 和 ¹H 核磁共振确定了化合物的结构。在 50、100、150 和 200 μg mL⁻¹的浓度下，在体外评估了这两种化合物对南方根结线虫卵和幼虫（J2）的活性。在 120 小时的孵育过程中，测试的两种化合物的累积死亡率和孵化抑制率均在 20%至 100%之间，孵化抑制率在 15%至 95.0%之间。在番茄品种 Riogrande 的盆栽试验中，两种化合物以 100、200 和 300 mg/kg 的浓度作为土壤淋溶处理，显著降低了线虫和植物生长参数。正二十九烷-10-醇和 23a-同型麦角甾-5-烯-3β-醇分别降低了根结数量（42.6 和 60.3）、根结指数（1.6 和 2.8）、每克根的雌虫数（37.3 和 57.0）、每克根的卵数（991.3 和 1273.0）、繁殖系数（Rf）（0.1 和 0.2）和根鲜重（14.33 和 17.0 g）在 300 mg/kg 浓度下，分别增加了根鲜重（49.0 和 48.4 g）、茎干鲜重（28.0 和 25.3 g）和株高（53.5 和 49.6 cm）。这些化合物可以为寻找新的南方根结线虫杀线虫剂提供新的思路。
