National Research Centre for the Working Environment, Copenhagen Ø., Denmark.
Reprod Toxicol. 2013 Nov;41:86-97. doi: 10.1016/j.reprotox.2013.05.006. Epub 2013 May 25.
We studied the effects of preconceptional exposure to multiwalled carbon nanotubes (MWCNTs): mature, female C57BL/6J mice were intratracheally instilled with 67μg NM-400 MWCNT, and the following day co-housed with mature males, in breeding pairs. Time to delivery of the first litter, litter parameters, maternal inflammation and histopathology of lung and liver were recorded. In male offspring, locomotor activity, startle response, and daily sperm production (DSP) were assessed. In the dams, lung and liver bore evidence of MWCNT exposure when assessed 6 weeks and 4 months after exposure. A short delay in the delivery of the first litter was observed in exposed females. Litter parameters, behavior and DSP were similar in control and exposed groups. In conclusion, instillation of a single dose of MWCNT induced long lasting pathological changes in dam lung and liver. Theoretically, lung inflammation due to particle exposure could interfere with female reproductive parameters. Whether the observed lag in delivery of a first litter was in fact caused by exposure to MWCNT should be addressed in a study designed specifically to elucidate effects on the early processes involved in establishment of pregnancy. Exposure was not associated with changes in the assessed gestational or offspring parameters.
我们研究了孕前接触多壁碳纳米管（MWCNTs）的影响：成熟的雌性 C57BL/6J 小鼠经气管内滴注 67μg NM-400 MWCNT，并于次日与成熟雄性同笼饲养。记录首次产仔的时间、产仔参数、母鼠炎症以及肺和肝的组织病理学变化。在雄性后代中，评估了运动活性、惊跳反应和每日精子产量（DSP）。在接触后 6 周和 4 个月时，暴露组母鼠的肺和肝中出现了 MWCNT 暴露的证据。暴露组母鼠首次产仔的时间略有延迟。对照组和暴露组的产仔参数、行为和 DSP 相似。总之，单次滴注 MWCNT 可诱导母鼠肺部和肝脏的长期病理变化。理论上，由于颗粒暴露引起的肺部炎症可能会干扰雌性生殖参数。实际上，首次产仔的延迟是否是由于暴露于 MWCNT 引起的，应该在一项专门设计的研究中阐明，该研究旨在阐明对妊娠早期过程的影响。暴露与评估的妊娠或后代参数的变化无关。
