Choudhury M A, Zaman M, Mollah A H, Hoque M A, Fatmi L E, Islam M N, Bhuiyan K J, Hossain M A
Department of Paediatric Haematology & Oncology, Mymensingh Medical College (MMC), Mymensingh, Bangladesh.
Mymensingh Med J. 2013 Apr;22(2):275-80.
This hospital based cross sectional analytic study was carried out in department of Pediatrics Dhaka Medical College Hospital and Combined Military Hospital from July 2009 to June 2010 to find out the relationship of iron status with febrile seizure in children. Sixty children aged 6 months to 5 years having febrile seizure were enrolled as cases after taking proper consent by purposive sampling. Sixty age matched children with fever but no seizures were taken as control after taking proper consent. Children having a febrile seizure, developmental delay, hemorrhagic disorder, severe malnutrition or micronutrient deficiency and abnormal CSF findings were excluded from the study. In cases after control of seizure 2 ml of CSF was taken for cytology, biochemistry, Gram & AFB staining. Two milliliter of blood from cases as well as control was collected, released to EDTA bottle for Hb, MCV and MCHC. Again 2 ml of blood was taken in plain test tube for testing serum iron, TIBC and for serum ferritin. Serum iron, TIBC, serum ferritin was estimated by automated analyzer (PENTRA for serum iron, DADE BEHRING for TIBC, IMMULITE 1000 for serum ferritin). There was no significant difference between two groups regarding age (p>0.05), sex (p>0.05). High fever (p<0.001) and continuous fever (p<0.001) was significantly associated with febrile seizure. Seizure was generalized in all with positive family history in 83.3% cases. The Mean±SD of blood Hb (Case 9.8±1.6; Control 10.7±0.9), MCH (Case 22.1±2.3; Control 24.3±3.6) & serum ferritin (Case 55.1±29.3; Control 99.6±81.9) level were significantly low associated with febrile seizure (p<0.05). No significant difference was found between cases and control regarding MCV, serum iron and serum TIBC.
这项基于医院的横断面分析研究于2009年7月至2010年6月在达卡医学院医院儿科和联合军事医院开展，旨在找出儿童铁状态与热性惊厥之间的关系。通过目的抽样法，在获得适当同意后，纳入了60名年龄在6个月至5岁的热性惊厥儿童作为病例。在获得适当同意后，选取了60名年龄匹配、发热但无惊厥的儿童作为对照。患有热性惊厥、发育迟缓、出血性疾病、严重营养不良或微量营养素缺乏以及脑脊液检查结果异常的儿童被排除在研究之外。在病例惊厥得到控制后，采集2毫升脑脊液用于细胞学、生物化学、革兰氏染色和抗酸杆菌染色。从病例组和对照组中各采集2毫升血液，放入乙二胺四乙酸（EDTA）瓶中用于检测血红蛋白（Hb）、平均红细胞体积（MCV）和平均红细胞血红蛋白浓度（MCHC）。再采集2毫升血液放入普通试管中用于检测血清铁、总铁结合力（TIBC）和血清铁蛋白。血清铁、TIBC和血清铁蛋白通过自动分析仪进行检测（血清铁用PENTRA检测，TIBC用DADE BEHRING检测，血清铁蛋白用IMMULITE 1000检测）。两组在年龄（p>0.05）、性别（p>0.05）方面无显著差异。高热（p<0.001）和持续发热（p<0.001）与热性惊厥显著相关。所有病例惊厥均为全身性，83.3%的病例有阳性家族史。血液中血红蛋白（病例组9.8±1.6；对照组10.7±0.9）、平均红细胞血红蛋白含量（病例组22.1±2.3；对照组24.3±3.6）和血清铁蛋白（病例组55.1±29.3；对照组99.6±81.9）水平的均值±标准差与热性惊厥显著相关（p<0.05）。病例组和对照组在MCV、血清铁和血清TIBC方面未发现显著差异。
