Department of Thoracic Surgery, The First Hospital of China Medical University, Heping, Shenyang, Liaoning 110001, PR China.
Int J Oncol. 2013 Aug;43(2):521-30. doi: 10.3892/ijo.2013.1967. Epub 2013 May 27.
Lung cancer is the most commonly diagnosed cancer worldwide. Loss of KISS1 expression has been associated with progression and poor prognosis of various cancers, however, the precise role of KISS1 expression in non-small cell lung cancer (NSCLC) is not well defined. KISS1 receptor (KISS1R, also named GPR54) coupled to KISS1, has been shown to play a pivotal role in suppressing cancer metastasis. In this study, 56 NSCLC specimens were divided into stage IIIB (locally advanced) and stage IV (metastatic). The mRNA and protein levels of KISS1 and KISS1R in cancer tissues were found to be lower compared to that in normal tissues using RT-PCR and western blot analysis, respectively. In addition, the expression of both KISS1 and KISS1R in stage IV NSCLC was lower compared to that in stage IIIB stage NSCLC. The cumulative survival rate of the patients with KISS1 or KISS1R expression was significantly higher compared to that without expression. KISS1 or KISS1R expression in NSCLC can be used to indicate favorable prognosis for disease outcome. Metastin, the product of the KISS1 gene, was lower in the serum of patients with stage IV NSCLC compared to that in stage IIIB NSCLC.
肺癌是全球最常见的癌症。KISS1 表达缺失与多种癌症的进展和预后不良有关，然而，KISS1 在非小细胞肺癌（NSCLC）中的确切作用尚未明确。KISS1 受体（KISS1R，也称为 GPR54）与 KISS1 偶联，已被证明在抑制癌症转移中发挥关键作用。在这项研究中，56 例 NSCLC 标本分为 IIIB 期（局部晚期）和 IV 期（转移性）。使用 RT-PCR 和 Western blot 分析分别发现，与正常组织相比，癌症组织中的 KISS1 和 KISS1R 的 mRNA 和蛋白水平较低。此外，与 IIIB 期 NSCLC 相比，IV 期 NSCLC 中 KISS1 和 KISS1R 的表达水平较低。KISS1 或 KISS1R 表达的患者的累积生存率明显高于无表达的患者。KISS1 或 KISS1R 在 NSCLC 中的表达可用于指示疾病结局的良好预后。转移性 KISS1 基因产物在 IV 期 NSCLC 患者的血清中低于 IIIB 期 NSCLC 患者。
