棘阿米巴在电场中的迁移。
Acanthamoeba migration in an electric field.
机构信息
Department of Dermatology, Institute for Regenerative Cures, School of Medicine, University of California at Davis, Davis, California, USA.
出版信息
Invest Ophthalmol Vis Sci. 2013 Jun 21;54(6):4225-33. doi: 10.1167/iovs.13-11968.
PURPOSE
We investigated the in vitro response of Acanthamoeba trophozoites to electric fields (EFs).
METHODS
Acanthamoeba castellanii were exposed to varying strengths of an EF. During EF exposure, cell migration was monitored using an inverted microscope equipped with a CCD camera and the SimplePCI 5.3 imaging system to capture time-lapse images. The migration of A. castellanii trophozoites was analyzed and quantified with ImageJ software. For analysis of cell migration in a three-dimensional culture system, Acanthamoeba trophozoites were cultured in agar, exposed to an EF, digitally video recorded, and analyzed at various Z focal planes.
RESULTS
Acanthamoeba trophozoites move at random in the absence of an EF, but move directionally in response to an EF. Directedness in the absence of an EF is 0.08 ± 0.01, while in 1200 mV/mm EF, directedness is significantly higher at -0.65 ± 0.01 (P < 0.001). We find that the trophozoite migration response is voltage-dependent, with higher directionality with higher voltage application. Acanthamoeba move directionally in a three-dimensional (3D) agar system as well when exposed to an EF.
CONCLUSIONS
Acanthamoeba trophozoites move directionally in response to an EF in a two-dimensional and 3D culture system. Acanthamoeba trophozoite migration is also voltage-dependent, with increased directionality with increasing voltage. This may provide new treatment modalities for Acanthamoeba keratitis.
目的
我们研究了棘阿米巴滋养体对电场（EF）的体外反应。
方法
棘阿米巴 castellanii 暴露于不同强度的 EF 中。在 EF 暴露期间，使用配备 CCD 相机和 SimplePCI 5.3 成像系统的倒置显微镜监测细胞迁移，以捕获延时图像。使用 ImageJ 软件分析和量化棘阿米巴 castellanii 滋养体的迁移。为了分析三维培养系统中的细胞迁移，将棘阿米巴滋养体在琼脂中培养，暴露于 EF 中，进行数字化视频记录，并在不同 Z 焦平面进行分析。
结果
在没有 EF 的情况下，棘阿米巴滋养体随机移动，但在 EF 的作用下会定向移动。在没有 EF 的情况下，定向性为 0.08 ± 0.01，而在 1200 mV/mm EF 中，定向性显著更高，为-0.65 ± 0.01（P < 0.001）。我们发现，滋养体迁移反应是电压依赖性的，随着电压的升高，定向性更高。当暴露于 EF 时，棘阿米巴在三维（3D）琼脂系统中也会定向移动。
结论
棘阿米巴滋养体在二维和 3D 培养系统中会对 EF 定向移动。棘阿米巴滋养体的迁移也是电压依赖性的，随着电压的增加，定向性增加。这可能为棘阿米巴角膜炎提供新的治疗方法。
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