huCdc7 在结直肠癌中的表达。
Expression of huCdc7 in colorectal cancer.
机构信息
Department of Oncology, Kunshan Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Kunshan 215300, Jiangsu Province, China.
出版信息
World J Gastroenterol. 2013 May 28;19(20):3130-3. doi: 10.3748/wjg.v19.i20.3130.
Abstract
AIM
To detect the expression of huCdc7 in colorectal cancer.
METHODS
The mRNA and protein expression of huCdc7 in 39 colorectal cancer tissue specimens and matched tumor-adjacent normal colorectal tissue specimens was detected by reverse transcription-polymerase chain reaction and immunohistochemistry, respectively.
RESULTS
The relative expression level of huCdc7 mRNA in colorectal cancer was significantly higher than that in tumor-adjacent normal colorectal tissues (0.03675 ± 1.00 vs 0.01199 ± 0.44, P < 0.05). huCdc7-positive cells displayed brown granules in the nucleus. Tumor tissues contained many huCdc7-positive cells, whereas normal colorectal tissues contained very few positive cells.
CONCLUSION
huCdc7 may play an important role in the development and progression of colorectal cancer.
摘要
目的
检测 huCdc7 在结直肠癌中的表达。
方法
采用逆转录-聚合酶链反应和免疫组织化学方法分别检测 39 例结直肠癌组织标本及其配对的癌旁正常结直肠组织标本中 huCdc7 的 mRNA 和蛋白表达。
结果
结直肠癌组织中 huCdc7 mRNA 的相对表达水平明显高于癌旁正常结直肠组织（0.03675 ± 1.00 比 0.01199 ± 0.44，P < 0.05）。huCdc7 阳性细胞的细胞核呈现棕色颗粒。肿瘤组织含有大量的 huCdc7 阳性细胞，而正常结直肠组织中阳性细胞很少。
结论
huCdc7 可能在结直肠癌的发生和发展中发挥重要作用。
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