Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Korea.
J Ginseng Res. 2011 Sep;35(3):301-7. doi: 10.5142/jgr.2011.35.3.301.
To evaluate the difference in expressing pharmacological effects of ginseng by intestinal microflora between Koreans, metabolic activities of ginseng, ginsenoside Rb1 and Rg1 by 100 fecal specimens were measured. The β-glucosidase activity for p-nitrophenyl- β-D-glucopyranoside was 0 to 0.42 μmol/min/mg and its average activity (mean±SD) was 0.10±0.07 μmol/min/mg. The metabolic activities of ginsenosides Rb1 and Rg1 were 0.01 to 0.42 and 0.01 to 0.38 pmol/min/mg, respectively. Their average activities were 0.25±0.08 and 0.15±0.09 pmol/min/mg, respectively. The compound K-forming activities from ginsenoside Rb1 and ginseng extract were 0 to 0.11 and 0 to 0.02 pmol/min/mg, respectively. Their average compound K-forming activities were 0.24±0.09 pmol/min/ mg and 2.14±3.66 fmol/min/mg, respectively. These activities all were not different between males and females, or between ages. Although compound K-forming activity from the aqueous extract of ginseng was low compared to that from ginenoside Rb1, their profiles were similar to those of isolated compounds. Based on these findings, we believe that the intestinal bacterial metabolic activities of ginseng components are variable in individuals and may be used as selection markers for responders to ginseng.
为了评估韩国人和肠道菌群对人参药效表达的差异，我们测量了 100 份粪便样本中人参、人参皂苷 Rb1 和 Rg1 的代谢活性。对 p-硝基苯-β-D-吡喃葡萄糖苷的β-葡萄糖苷酶活性为 0 至 0.42μmol/min/mg，其平均活性（均值±SD）为 0.10±0.07μmol/min/mg。人参皂苷 Rb1 和 Rg1 的代谢活性分别为 0.01 至 0.42 和 0.01 至 0.38 pmol/min/mg，其平均活性分别为 0.25±0.08 和 0.15±0.09 pmol/min/mg。从人参皂苷 Rb1 和人参提取物中生成的化合物 K 的活性分别为 0 至 0.11 和 0 至 0.02 pmol/min/mg，其平均化合物 K 生成活性分别为 0.24±0.09 pmol/min/mg 和 2.14±3.66 fmol/min/mg。这些活性在男性和女性之间，或在年龄之间均无差异。虽然与人参皂苷 Rb1 相比，人参水提取物生成化合物 K 的活性较低，但它们的谱图与分离化合物的谱图相似。基于这些发现，我们认为人参成分的肠道细菌代谢活性在个体之间是可变的，可作为人参应答者的选择标记。
