用光显微镜绘制大脑回路。
Mapping brain circuitry with a light microscope.
机构信息
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA.
出版信息
Nat Methods. 2013 Jun;10(6):515-23. doi: 10.1038/nmeth.2477.
Abstract
The beginning of the 21st century has seen a renaissance in light microscopy and anatomical tract tracing that together are rapidly advancing our understanding of the form and function of neuronal circuits. The introduction of instruments for automated imaging of whole mouse brains, new cell type–specific and trans-synaptic tracers, and computational methods for handling the whole-brain data sets has opened the door to neuroanatomical studies at an unprecedented scale. We present an overview of the present state and future opportunities in charting long-range and local connectivity in the entire mouse brain and in linking brain circuits to function.
摘要
21 世纪初，光学显微镜和解剖学轨迹追踪技术复兴，两者共同快速推进了我们对神经元回路的形态和功能的理解。用于自动成像整个小鼠大脑的仪器、新型细胞类型特异性和跨突触示踪剂以及用于处理全脑数据集的计算方法的出现，为神经解剖学研究打开了前所未有的规模之门。我们概述了在整个小鼠大脑中绘制长程和局部连接图以及将大脑回路与功能联系起来的现状和未来机会。
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