School of Mathematics and Physics, The University of Queensland, St Lucia, QLD 4072, Australia.
Opt Lett. 2013 Jun 1;38(11):1847-9. doi: 10.1364/OL.38.001847.
We demonstrate a way of contrast increasing in wide-field optical images of diamond nanocrystals containing nitrogen-vacancy (NV) centers surrounded by an autofluorescing material, as in the case of intercellular imaging. Subtraction of an image taken in the presence of an external magnetic field of about 25 mT from an image of the same area measured when the field was absent eliminates the background and enhances the contrast because luminescence of the NV centers decreases in the presence of the field while the auto luminescence is not affected.
我们展示了一种在含有氮空位（NV）中心的金刚石纳米晶体的宽场光学图像中增加对比度的方法，这种方法适用于细胞间成像，其中 NV 中心被自发荧光材料包围。从在约 25 mT 的外磁场存在下拍摄的图像中减去在磁场不存在时测量的同一区域的图像，可以消除背景并提高对比度，因为 NV 中心的发光在磁场存在下减少，而自发荧光不受影响。
