Yamamoto T, Seino Y, Fukumoto H, Koh G, Yano H, Inagaki N, Yamada Y, Inoue K, Manabe T, Imura H
Division of Metabolism and Clinical Nutrition, Kyoto University School of Medicine, Japan.
Biochem Biophys Res Commun. 1990 Jul 16;170(1):223-30. doi: 10.1016/0006-291x(90)91263-r.
The expression of five facilitative glucose transporter genes, GLUT1 (erythrocyte type), GLUT2 (liver type), GLUT3 (brain type), GLUT4 (muscle/fat type), and GLUT5 (small intestine type), was examined in human cancer tissues of the digestive system by RNA blotting analysis. The amounts of the GLUT1, GLUT2, and GLUT3 transcripts were elevated in most cancer tissues studied, although the expression of the GLUT2 gene is primarily restricted to the liver. On the other hand, mRNA levels of GLUT4 and GLUT5 were below sensitivity in all cancer tissues examined. These results suggest that over-expression of GLUT1 and GLUT3 might be closely related with tissue development and that the acceleration of glucose uptake by transformed cells may result, at least in part, from the increase in the expression of these two glucose transporters.
通过RNA印迹分析，检测了五种易化型葡萄糖转运蛋白基因，即GLUT1（红细胞型）、GLUT2（肝型）、GLUT3（脑型）、GLUT4（肌肉/脂肪型）和GLUT5（小肠型）在人类消化系统癌组织中的表达情况。尽管GLUT2基因的表达主要局限于肝脏，但在大多数所研究的癌组织中，GLUT1、GLUT2和GLUT3转录本的量均有所升高。另一方面，在所有检测的癌组织中，GLUT4和GLUT5的mRNA水平均低于检测灵敏度。这些结果表明，GLUT1和GLUT3的过表达可能与组织发育密切相关，并且转化细胞对葡萄糖摄取的加速可能至少部分是由于这两种葡萄糖转运蛋白表达的增加所致。
