Yamauchi T, Hiraiwa M, Kobayashi H, Uda Y, Miyatake T, Tsuji S
Department of Neurology, Niigata University, Japan.
Biochem Biophys Res Commun. 1990 Jul 16;170(1):231-7. doi: 10.1016/0006-291x(90)91264-s.
Two species of cDNAs for human alpha-N-acetylgalactosaminidase were isolated from a human fibroblast cDNA library. The two species differ each other by a 70 bp insertion in the coding region. Transient expression study in COS cells demonstrated that only the cDNA without the 70 bp insertion expressed alpha-N-acetylgalactosaminidase activity. Analysis of mRNA species utilizing polymerase chain reaction revealed that the majority of the mRNA does not contain the 70 bp insertion, and the mRNA containing the 70 bp insertion is present only in a minor amount in human brain.
从人成纤维细胞cDNA文库中分离出两种人α-N-乙酰半乳糖胺酶的cDNA。这两种cDNA在编码区相差70bp的插入片段。在COS细胞中的瞬时表达研究表明，只有没有70bp插入片段的cDNA表达α-N-乙酰半乳糖胺酶活性。利用聚合酶链反应对mRNA种类进行分析发现，大多数mRNA不包含70bp插入片段，而包含70bp插入片段的mRNA在人脑内仅少量存在。
