University of Southern California, 1050 Childs Way, RRI 201, Los Angeles, CA 90089-2910.
J Gerontol A Biol Sci Med Sci. 2014 Mar;69(3):253-9. doi: 10.1093/gerona/glt078. Epub 2013 May 30.
The cytoplasmic chaperone gene Hsp70 and the mitochondrial chaperone gene Hsp22 are upregulated during normal aging in Drosophila in tissue-general patterns. In addition, Hsp22 reporters are dramatically upregulated during aging in a subset of the oenocytes (liver-like cells). Hsp22 reporter expression varied dramatically between individual oenocytes and between groups of oenocytes located in adjacent body segments, and was negatively correlated with accumulation of age pigment, indicating cell-specific and cell-lineage-specific patterns of oenocyte aging. Conditional transgenic systems were used to express 88 transgenes to search for trans-regulators of the Hsp70 and Hsp22 reporters during aging. The wingless gene increased tissue-general upregulation of both Hsp70 and Hsp22 reporters. In contrast, the mitochondrial genes MnSOD and Hsp22 increased expression of Hsp22 reporters in the oenocytes and decreased accumulation of age pigment in these cells. The data suggest that cell-specific and cell lineage-specific patterns of mitochondrial malfunction contribute to oenocyte aging.
细胞质伴侣基因 Hsp70 和线粒体伴侣基因 Hsp22 在果蝇的正常衰老过程中以组织普遍的模式上调。此外，Hsp22 报告基因在一部分卵母细胞（类肝细胞）的衰老过程中显著上调。Hsp22 报告基因的表达在个体卵母细胞之间以及位于相邻体节的卵母细胞群之间差异很大，与年龄色素的积累呈负相关，表明卵母细胞衰老具有细胞特异性和细胞谱系特异性模式。条件性转基因系统被用于表达 88 个转基因，以寻找在衰老过程中调节 Hsp70 和 Hsp22 报告基因的转录调节剂。无翅基因增加了 Hsp70 和 Hsp22 报告基因在组织中的普遍上调。相比之下，线粒体基因 MnSOD 和 Hsp22 增加了卵母细胞中 Hsp22 报告基因的表达，并减少了这些细胞中年龄色素的积累。数据表明，线粒体功能障碍的细胞特异性和细胞谱系特异性模式导致了卵母细胞的衰老。
