• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

旁分泌和自分泌信号在脂肪干细胞成脂分化的早期阶段的作用。

The role of paracrine and autocrine signaling in the early phase of adipogenic differentiation of adipose-derived stem cells.

机构信息

Department of Micro- and Nanotechnology, Technical University of Denmark, Kgs. Lyngby, Denmark.

出版信息

PLoS One. 2013 May 28;8(5):e63638. doi: 10.1371/journal.pone.0063638. Print 2013.

DOI:10.1371/journal.pone.0063638
PMID:23723991
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3665830/
Abstract

INTRODUCTION

High cell density is known to enhance adipogenic differentiation of mesenchymal stem cells, suggesting secretion of signaling factors or cell-contact-mediated signaling. By employing microfluidic biochip technology, we have been able to separate these two processes and study the secretion pathways.

METHODS AND RESULTS

Adipogenic differentiation of human adipose-derived stem cells (ASCs) cultured in a microfluidic system was investigated under perfusion conditions with an adipogenic medium or an adipogenic medium supplemented with supernatant from differentiating ASCs (conditioned medium). Conditioned medium increased adipogenic differentiation compared to adipogenic medium with respect to accumulation of lipid-filled vacuoles and gene expression of key adipogenic markers (C/EBPα, C/EBPβ, C/EBPδ, PPARγ, LPL and adiponectin). The positive effects of conditioned medium were observed early in the differentiation process.

CONCLUSIONS

Using different cell densities and microfluidic perfusion cell cultures to suppress the effects of cell-released factors, we have demonstrated the significant role played by auto- or paracrine signaling in adipocyte differentiation. The cell-released factor(s) were shown to act in the recruitment phase of the differentiation process.

摘要

简介

高细胞密度已知可增强间充质干细胞的成脂分化，提示分泌信号因子或细胞接触介导的信号。通过采用微流控生物芯片技术，我们能够将这两个过程分开，并研究分泌途径。

方法和结果

在灌注条件下，用人脂肪来源的干细胞（ASCs）在微流控系统中培养的成脂分化，使用含成脂培养基或含分化 ASCs（条件培养基）上清液的成脂培养基进行研究。与成脂培养基相比，条件培养基增加了脂滴填充空泡的积累和关键成脂标志物（C/EBPα、C/EBPβ、C/EBPδ、PPARγ、LPL 和脂联素）的基因表达，从而增加了成脂分化。条件培养基的积极作用在分化过程的早期就观察到了。

结论

通过使用不同的细胞密度和微流控灌注细胞培养来抑制细胞释放因子的影响，我们证明了自分泌或旁分泌信号在脂肪细胞分化中发挥了重要作用。证明细胞释放的因子在分化过程的招募阶段起作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a017/3665830/fc6017a1a174/pone.0063638.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a017/3665830/2646d27f2d4c/pone.0063638.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a017/3665830/0e4ef6f5f9c0/pone.0063638.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a017/3665830/f2235335ff90/pone.0063638.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a017/3665830/50041e972c1c/pone.0063638.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a017/3665830/b8caedb16864/pone.0063638.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a017/3665830/9ec14c9a2a1e/pone.0063638.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a017/3665830/fc6017a1a174/pone.0063638.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a017/3665830/2646d27f2d4c/pone.0063638.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a017/3665830/0e4ef6f5f9c0/pone.0063638.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a017/3665830/f2235335ff90/pone.0063638.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a017/3665830/50041e972c1c/pone.0063638.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a017/3665830/b8caedb16864/pone.0063638.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a017/3665830/9ec14c9a2a1e/pone.0063638.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a017/3665830/fc6017a1a174/pone.0063638.g007.jpg

相似文献

1
The role of paracrine and autocrine signaling in the early phase of adipogenic differentiation of adipose-derived stem cells.旁分泌和自分泌信号在脂肪干细胞成脂分化的早期阶段的作用。
PLoS One. 2013 May 28;8(5):e63638. doi: 10.1371/journal.pone.0063638. Print 2013.
2
Ginsenoside Rg1 Accelerates Paracrine Activity and Adipogenic Differentiation of Human Breast Adipose-Derived Stem Cells in a Dose-Dependent Manner In Vitro.体外实验中，人参皂苷 Rg1 以剂量依赖的方式加速人乳腺脂肪来源干细胞的旁分泌活性和脂肪生成分化。
Cell Transplant. 2019 Mar;28(3):286-295. doi: 10.1177/0963689719825615. Epub 2019 Jan 24.
3
The differentiation of human adipose-derived stem cells towards a urothelium-like phenotype in vitro and the dynamic temporal changes of related cytokines by both paracrine and autocrine signal regulation.人脂肪来源干细胞在体外向尿路上皮样表型的分化及旁分泌和自分泌信号调节相关细胞因子的动态时程变化。
PLoS One. 2014 Apr 21;9(4):e95583. doi: 10.1371/journal.pone.0095583. eCollection 2014.
4
EGF and hydrocortisone as critical factors for the co-culture of adipogenic differentiated ASCs and endothelial cells.表皮生长因子（EGF）和氢化可的松是脂肪生成分化的脂肪干细胞（ASCs）与内皮细胞共培养的关键因素。
Differentiation. 2017 May-Jun;95:21-30. doi: 10.1016/j.diff.2017.01.002. Epub 2017 Jan 20.
5
Novel Neohesperidin Dihydrochalcone Analogue Inhibits Adipogenic Differentiation of Human Adipose-Derived Stem Cells through the Nrf2 Pathway.新型橙皮苷二氢查尔酮类似物通过 Nrf2 通路抑制人脂肪干细胞的成脂分化。
Int J Mol Sci. 2018 Jul 29;19(8):2215. doi: 10.3390/ijms19082215.
6
DHL-HisZn, a novel antioxidant, enhances adipogenic differentiation and antioxidative response in adipose-derived stem cells.新型抗氧化剂DHL-HisZn可增强脂肪来源干细胞的成脂分化和抗氧化反应。
Biomed Pharmacother. 2016 Dec;84:1601-1609. doi: 10.1016/j.biopha.2016.10.066. Epub 2016 Nov 5.
7
Gap junctional intercellular communication in adipose-derived stromal/stem cells is cell density-dependent and positively impacts adipogenic differentiation.脂肪来源的基质/干细胞中的缝隙连接细胞间通讯依赖于细胞密度，并正向影响脂肪生成分化。
J Cell Physiol. 2018 Apr;233(4):3315-3329. doi: 10.1002/jcp.26178. Epub 2017 Oct 27.
8
Novel pathway of adipogenesis through cross-talk between adipose tissue macrophages, adipose stem cells and adipocytes: evidence of cell plasticity.脂肪组织巨噬细胞、脂肪干细胞和脂肪细胞之间的对话形成新的脂肪生成途径：细胞可塑性的证据。
PLoS One. 2011 Mar 31;6(3):e17834. doi: 10.1371/journal.pone.0017834.
9
MicroRNA-138 Suppresses Adipogenic Differentiation in Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting Lipoprotein Lipase.MicroRNA-138 通过靶向脂蛋白脂肪酶抑制人脂肪组织来源的间充质干细胞的成脂分化。
Yonsei Med J. 2019 Dec;60(12):1187-1194. doi: 10.3349/ymj.2019.60.12.1187.
10
IFATS collection: Adipose stromal cell differentiation is reduced by endothelial cell contact and paracrine communication: role of canonical Wnt signaling.国际脂肪治疗与科学联合会（IFATS）文献汇编：内皮细胞接触和旁分泌通讯可降低脂肪基质细胞分化：经典Wnt信号通路的作用
Stem Cells. 2008 Oct;26(10):2674-81. doi: 10.1634/stemcells.2008-0277. Epub 2008 Jul 31.

引用本文的文献

1
Fabrication of a novel 3D-printed perfusion bioreactor for complex cell culture models.用于复杂细胞培养模型的新型3D打印灌注生物反应器的制造。
Sci Rep. 2025 Mar 24;15(1):10134. doi: 10.1038/s41598-025-94093-z.
2
Aberrant Wnt/β-catenin signaling in the mesenchymal stem cells of LZTFL1-depleted mice leads to increased adipogenesis, with implications for obesity.LZTFL1缺失小鼠间充质干细胞中异常的Wnt/β-连环蛋白信号传导导致脂肪生成增加，这与肥胖有关。
J Biol Chem. 2025 Jan;301(1):108057. doi: 10.1016/j.jbc.2024.108057. Epub 2024 Dec 9.
3
Mesenchymal Stem Cell in Pancreatic Islet Transplantation.

本文引用的文献

1
Prostaglandins as PPARγ Modulators in Adipogenesis.前列腺素作为脂肪生成中的 PPARγ 调节剂。
PPAR Res. 2012;2012:527607. doi: 10.1155/2012/527607. Epub 2012 Dec 25.
2
The MainSTREAM component platform: a holistic approach to microfluidic system design.主流组件平台：微流体系统设计的整体方法。
J Lab Autom. 2013 Jun;18(3):212-28. doi: 10.1177/2211068212461445. Epub 2012 Sep 26.
3
The effect of culture conditions on the adipogenic and osteogenic inductions of mesenchymal stem cells on micropatterned surfaces.培养条件对微图案化表面间充质干细胞成脂和成骨诱导的影响。
间充质干细胞在胰岛移植中的应用
Biomedicines. 2023 May 11;11(5):1426. doi: 10.3390/biomedicines11051426.
4
A quantitative meta-analysis comparing cell models in perfused organ on a chip with static cell cultures.定量荟萃分析比较在灌注器官芯片中的细胞模型与静态细胞培养。
Sci Rep. 2023 May 22;13(1):8233. doi: 10.1038/s41598-023-35043-5.
5
Epstein-Barr virus-induced gene 3 commits human mesenchymal stem cells to differentiate into chondrocytes via endoplasmic reticulum stress sensor.EB 病毒诱导基因 3 通过内质网应激传感器促使人类间充质干细胞向软骨细胞分化。
PLoS One. 2022 Dec 22;17(12):e0279584. doi: 10.1371/journal.pone.0279584. eCollection 2022.
6
MYH10 Governs Adipocyte Function and Adipogenesis through Its Interaction with GLUT4.MYH10 通过与 GLUT4 相互作用来调节脂肪细胞功能和脂肪生成。
Int J Mol Sci. 2022 Feb 21;23(4):2367. doi: 10.3390/ijms23042367.
7
Slow diffusion on the monolayer culture enhances auto/paracrine effects of Noggin in differentiation of human iPS cells induced by BMP.单层培养中的缓慢扩散增强了Noggin在BMP诱导的人诱导多能干细胞分化中的自分泌/旁分泌作用。
Biochem Biophys Rep. 2021 Dec 27;29:101195. doi: 10.1016/j.bbrep.2021.101195. eCollection 2022 Mar.
8
Mesenchymal Stem/Stromal Cells Derived from Human and Animal Perinatal Tissues-Origins, Characteristics, Signaling Pathways, and Clinical Trials.人及动物围生期组织来源的间充质干细胞：起源、特性、信号通路及临床试验。
Cells. 2021 Nov 23;10(12):3278. doi: 10.3390/cells10123278.
9
Application of a deep learning-based image analysis and live-cell imaging system for quantifying adipogenic differentiation kinetics of adipose-derived stem/stromal cells.基于深度学习的图像分析和活细胞成像系统在定量脂肪来源干细胞/基质细胞成脂分化动力学中的应用。
Adipocyte. 2021 Dec;10(1):621-630. doi: 10.1080/21623945.2021.2000696.
10
Inhibitory Effect of Bovine Adipose-Derived Mesenchymal Stem Cells on Lipopolysaccharide Induced Inflammation of Endometrial Epithelial Cells in Dairy Cows.牛脂肪间充质干细胞对脂多糖诱导的奶牛子宫内膜上皮细胞炎症的抑制作用
Front Vet Sci. 2021 Oct 21;8:726328. doi: 10.3389/fvets.2021.726328. eCollection 2021.
Biomaterials. 2012 Sep;33(26):6008-19. doi: 10.1016/j.biomaterials.2012.05.010. Epub 2012 Jun 7.
4
Murine 3T3-L1 adipocyte cell differentiation model: validated reference genes for qPCR gene expression analysis.鼠 3T3-L1 脂肪细胞分化模型：qPCR 基因表达分析的验证参考基因。
PLoS One. 2012;7(5):e37517. doi: 10.1371/journal.pone.0037517. Epub 2012 May 22.
5
Secreted factors from adipose tissue increase adipogenic differentiation of mesenchymal stem cells.脂肪组织分泌的因子可促进间充质干细胞的成脂分化。
Cell Prolif. 2012 Aug;45(4):311-9. doi: 10.1111/j.1365-2184.2012.00823.x. Epub 2012 May 10.
6
The WNT inhibitor Dickkopf 1 and bone morphogenetic protein 4 rescue adipogenesis in hypertrophic obesity in humans.WNT 抑制剂 Dickkopf-1 和骨形态发生蛋白 4 可挽救人类肥大性肥胖症中的脂肪生成。
Diabetes. 2012 May;61(5):1217-24. doi: 10.2337/db11-1419. Epub 2012 Mar 23.
7
Concise review: Adipose-derived stem cells as a novel tool for future regenerative medicine.简明综述：脂肪来源干细胞作为未来再生医学的一种新型工具。
Stem Cells. 2012 May;30(5):804-10. doi: 10.1002/stem.1076.
8
The transforming growth factor-beta/bone morphogenetic protein signalling pathway in adipogenesis.脂肪生成中的转化生长因子-β/骨形态发生蛋白信号通路。
Int J Biochem Cell Biol. 2012 Mar;44(3):475-9. doi: 10.1016/j.biocel.2011.12.014. Epub 2011 Dec 31.
9
Identification of BMP and activin membrane-bound inhibitor (BAMBI) as a potent negative regulator of adipogenesis and modulator of autocrine/paracrine adipogenic factors.鉴定骨形态发生蛋白和激活素的细胞膜结合抑制剂（BAMBI）作为脂肪生成的有效负调节剂和自分泌/旁分泌脂肪生成因子的调节剂。
Diabetes. 2012 Jan;61(1):124-36. doi: 10.2337/db11-0998.
10
A self-contained, programmable microfluidic cell culture system with real-time microscopy access.一种具有实时显微镜访问功能的独立可编程微流控细胞培养系统。
Biomed Microdevices. 2012 Apr;14(2):385-99. doi: 10.1007/s10544-011-9615-6.