Prakash Rajat, Juyal Deepak, Negi Vikrant, Pal Shekhar, Adekhandi Shamanth, Sharma Munesh, Sharma Neelam
Department of Microbiology and Immunology, Veer Chandra Singh Garhwali Government, Medical Sciences and Research Institute, Srinagar-Garhwal, Uttarakhand, India.
N Am J Med Sci. 2013 Apr;5(4):282-7. doi: 10.4103/1947-2714.110436.
Chronic suppurative otitis media (CSOM) is a notorious infection and a major health problem in developing countries causing serious local damage and threatening complications. Early and effective treatment based on the knowledge of causative micro-organisms and their antimicrobial sensitivity ensures prompt clinical recovery and possible complications can thus be avoided.
The aim of this study was to isolate the organisms associated with CSOM and to detect the antibiogram of the aerobic isolates.
A total of 204 patients clinically diagnosed of CSOM were enrolled in the study and the samples were obtained from each patient using sterile cotton swabs and cultured for microbial flora. Drug susceptibility testing for aerobic isolates was conducted using Kirby-Bauer disc diffusion method.
The most common causative organisms isolated were Staphylococcus aureus (48.69%) and Pseudomonas aeruginosa (19.89%) amongst the 191 aerobic isolates. Anaerobes accounted for 29.41% of the isolates while 12.25% were fungi. Antimicrobial profile of aerobic isolates revealed maximum sensitivity to amikacin (95.5%), ceftriaxone (83.4%) and gentamicin (82.7%).
Knowing the etiological agents of CSOM and their antimicrobial susceptibility is of essential importance for an efficient treatment, prevention of both complications and development of antibiotic resistance and finally, the reduction of the treatment costs.
慢性化脓性中耳炎(CSOM)是一种难治性感染,在发展中国家是一个主要的健康问题,会造成严重的局部损害并引发威胁生命的并发症。基于对致病微生物及其抗菌敏感性的了解进行早期有效治疗,可确保临床迅速康复,从而避免可能出现的并发症。
本研究旨在分离与CSOM相关的微生物,并检测需氧菌分离株的抗菌谱。
共有204例临床诊断为CSOM的患者纳入本研究,使用无菌棉签从每位患者获取样本并培养微生物菌群。采用 Kirby-Bauer 纸片扩散法对需氧菌分离株进行药敏试验。
在191株需氧菌分离株中,最常见的致病微生物是金黄色葡萄球菌(48.69%)和铜绿假单胞菌(19.89%)。厌氧菌占分离株的29.41%,真菌占12.25%。需氧菌分离株的抗菌谱显示对阿米卡星(95.5%)、头孢曲松(83.4%)和庆大霉素(82.7%)的敏感性最高。
了解CSOM的病原体及其抗菌敏感性对于有效治疗、预防并发症以及抗生素耐药性的产生,最终降低治疗成本至关重要。