Zhao Shan, Yao De-Sheng, Chen Jun-Ying, Ding Nan
Department of Gynecologic Oncology, the Affiliated Tumor Hospital of Guangxi Medical University, Nanning, China.
Asian Pac J Cancer Prev. 2013;14(4):2289-93. doi: 10.7314/apjcp.2013.14.4.2289.
MicroRNAs (miRNAs) are small, non-coding RNAs that are critical regulators of various diseases. MicroRNA- 20a (miR-20a) and microRNA-203 (miR-203) have previously shown significant alteration in a range of cancers. In this study, the expression levels of miR-20a and miR-203 in 100 cervical cancer tissues were detected by qRT-PCR and compared to patient matched-nontumor cervical tissues. Correlations between expression level and clinicopathologic characteristics of cervical cancer were also analyzed. Finally, we studied the effect of miR- 20a and miR-203 on cell proliferation in cervical cancer cell lines by MTT. We found that the expression level of miR-20a (P<0.001) was significantly higher in cervical cancer patients than in healthy controls, while that of miR-203 (P<0.001) was lower. Aberrant expression of miR-20a was correlated with lymph node metastasis (LNM), histological grade and tumor diameter, but down-regulated miR-203 was correlated with LNM only. Furthermore, we found that over-expression of miR-203 decreased cell proliferation, while reduction of miR- 20a also prevented tumor progression. Our results support the involvement of miR-20a and miR-203 in cervical tumorigenesis. We propose that miRNAs might be used as therapeutic agents for cervical cancer.
微小RNA（miRNA）是一类小的非编码RNA，是多种疾病的关键调节因子。微小RNA - 20a（miR - 20a）和微小RNA - 203（miR - 203）先前已显示在一系列癌症中存在显著改变。在本研究中，通过qRT - PCR检测了100例宫颈癌组织中miR - 20a和miR - 203的表达水平，并与患者匹配的非肿瘤宫颈组织进行比较。还分析了表达水平与宫颈癌临床病理特征之间的相关性。最后，我们通过MTT研究了miR - 20a和miR - 203对宫颈癌细胞系细胞增殖的影响。我们发现，宫颈癌患者中miR - 20a的表达水平（P<0.001）显著高于健康对照，而miR - 203的表达水平（P<0.001）则较低。miR - 20a的异常表达与淋巴结转移（LNM）、组织学分级和肿瘤直径相关，但miR - 203表达下调仅与LNM相关。此外，我们发现miR - 203的过表达降低了细胞增殖，而miR - 20a的减少也阻止了肿瘤进展。我们的结果支持miR - 20a和miR - 203参与宫颈肿瘤发生。我们提出，miRNA可能用作宫颈癌的治疗药物。
