Zhang Sheng-Chang, Huang Peng, Zhao Yong-Xiang, Liu Shu-Yan, He Shu-Jia, Xie Xiao-Xun, Luo Gou-Rong, Zhou Su-Fang
School of Pre-clinical Sciences, Guangxi Medical University, Nanning, China.
Asian Pac J Cancer Prev. 2013;14(4):2383-6. doi: 10.7314/apjcp.2013.14.4.2383.
Senescence marker protein 30 (SMP30), a hepatocellular carcinoma (HCC) associated antigen, was earlier shown by our research group to be highly expressed in HCC paracancerous tissues, but have low levels in HCC tissues. In order to detect anti-SMP30 antibody in serum of HCC patients, we established pET30a-SMP30 and pColdIII-SMP30 expression systems in Escherichia coli. However, the expression product was mainly in the form of inclusion bodies. In this research, we used several combinations of chaperones, four molecular chaperone plasmids with pET30a-SMP30 and five molecular chaperone plasmids with pColdIII-SMP30 to increase the amount of soluble protein. Results showed that co-expression of HIS-SMP30 with pTf16, combined with the addition of osmosis-regulator, and a two-step expression resulted in the highest enhancement of solubility. A total of 175 cases of HCC serum were studied by ELISA to detect anti- SMP30 antibody with recombinant SMP30 protein. Some 22 were positive and x2 two-sided tests all showed P>0.05, although it remained unclear whether there was a relationship between positive cases and clinical diagnostic data.
衰老标记蛋白30（SMP30）是一种与肝细胞癌（HCC）相关的抗原，我们的研究小组 earlier 发现它在肝癌癌旁组织中高表达，但在肝癌组织中水平较低。为了检测肝癌患者血清中的抗SMP30抗体，我们在大肠杆菌中建立了pET30a-SMP30和pColdIII-SMP30表达系统。然而，表达产物主要以包涵体的形式存在。在本研究中，我们使用了几种伴侣蛋白组合，四种与pET30a-SMP30的分子伴侣质粒和五种与pColdIII-SMP30的分子伴侣质粒来增加可溶性蛋白的量。结果表明，HIS-SMP30与pTf16共表达，结合添加渗透调节剂和两步表达，导致溶解度提高最多。通过ELISA用重组SMP30蛋白研究了175例肝癌血清，检测抗SMP30抗体。约22例为阳性，x2双侧检验均显示P>0.05，尽管阳性病例与临床诊断数据之间是否存在关系仍不清楚。
原文中“earlier”翻译为“earlier”有误，可能是“earlier”，正确翻译为“较早地” 。
