Gabriel B, Teissié J
Laboratoire de Pharmacologie et de Toxicologie Fondamentales du CNRS, Département III Glycoconjugués et Biomembranes, Toulouse, France.
Eur J Biochem. 1994 Jul 1;223(1):25-33. doi: 10.1111/j.1432-1033.1994.tb18962.x.
Cells can be permeabilized transiently when pulsed by a brief intense external electric field. The molecular and cellular bases of cell electropermeabilization are still unclear. This process can be described by a local transient membrane organisation in which high permeability exists. In this study, using the chemiluminescent probe lucigenin, we showed that electropulsation of Chinese hamster ovary cells induced generation of reactive oxygen species at the electropermeabilized cell level. This generation was directly associated with the part of the membrane surface which is electrically restructured, as shown by its dependence on electric parameters. The electroinduced cell process was activated by Ca2+ and Mg2+ ions, and by exogenous adenosine 5'-triphosphate. A metal-ion-catalyzed Haber-Weiss reaction was thought to occur in the process, as shown by the action of effector molecules of Haber-Weiss reaction such as the chelating agent EDTA, and the hydroxyl radical scavenger dimethylsulfoxide. The modulation of the oxygen species electroinduction and the use of antioxidant products (dimethylsulfoxide, sodium L-ascorbate) showed that cell survival after electric treatment was directly correlated to the oxidative jump intensity. This observation had to be associated with the cell-damaging action of oxygen-reactive species.
当受到短暂强烈的外部电场脉冲作用时，细胞可被瞬时通透化。细胞电通透化的分子和细胞基础仍不清楚。这一过程可用存在高通透性的局部瞬时膜组织来描述。在本研究中，我们使用化学发光探针光泽精，表明中国仓鼠卵巢细胞的电脉冲在电通透化细胞水平上诱导了活性氧的产生。如对电参数的依赖性所示，这种产生与膜表面发生电重构的部分直接相关。电诱导的细胞过程由Ca2+和Mg2+离子以及外源性腺苷5'-三磷酸激活。如螯合剂乙二胺四乙酸和羟基自由基清除剂二甲基亚砜等哈伯-维伊斯反应效应分子的作用所示，该过程中可能发生了金属离子催化的哈伯-维伊斯反应。氧物种电诱导的调节以及抗氧化产物（二甲基亚砜、L-抗坏血酸钠）的使用表明，电处理后的细胞存活与氧化跃升强度直接相关。这一观察结果必须与氧反应性物种对细胞的损伤作用联系起来。
