一种用于RNA核糖甲基化酶的快速检测技术。
A rapid assay technique for RNA ribose methylases.
作者信息
Svensson I, Patel V J
出版信息
Nucleic Acids Res. 1975 Apr;2(4):603-11. doi: 10.1093/nar/2.4.603.
Abstract
A rapid technique for quantitative separation of ribose-methylated nucleosides from base-methylated and non-methylated nucleosides by chromatography on DEAE-cellulose paper in the presence of borate is described. The method has been used as an assay for tRNA ribose methylases from yeast, using under methylated Escherichia coli tRNA as substrate. The main product formed with a partly purified yeast enzyme was characterized as 2'-O-methylcytidine.
摘要
本文描述了一种在硼酸盐存在下，通过DEAE - 纤维素纸色谱法从碱基甲基化和未甲基化的核苷中快速定量分离核糖甲基化核苷的技术。该方法已被用作检测酵母中tRNA核糖甲基化酶的分析方法，使用甲基化不足的大肠杆菌tRNA作为底物。用部分纯化的酵母酶形成的主要产物被鉴定为2'-O-甲基胞苷。
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本文引用的文献
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ON THE 2'-O-METHYLRIBONUCLEOSIDE CONTENT OF RIBONUCLEIC ACIDS.论核糖核酸的2'-O-甲基核糖核苷含量。
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Studies on microbial ribonucleic acid. IV. Two mutants of Saccharomyces cerevisiae lacking N-2-dimethylguanine in soluble ribonucleic acid.微生物核糖核酸的研究。IV。酿酒酵母的两个突变体，其可溶性核糖核酸中缺乏N-2-二甲基鸟嘌呤。
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Evidence for enzymatic methylation in vitro of the ribose moiety of RNA.RNA核糖部分体外酶促甲基化的证据。
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Primary structure of wheat germ phenylalanine transfer RNA.小麦胚苯丙氨酸转移核糖核酸的一级结构
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