利用海拉细胞核匀浆合成含甲基化封闭5'端的RNA 。
Synthesis of RNA containing a methylated blocked 5' terminus by HeLa nuclear homogenates.
作者信息
Groner Y, Hurwitz J
出版信息
Proc Natl Acad Sci U S A. 1975 Aug;72(8):2930-4. doi: 10.1073/pnas.72.8.2930.
Abstract
RNA synthesized in vitro by HeLa nuclei contains a variety of blocked methylated 5'-terminal structures that can be grouped into four general types: (a) m(7)GppN(m)p...; (b) m(7)GppN(m)pM(m)p...; (c) m(7)GpppN(m)p...; and (d) m(7)GpppN(m)pM(m).... Both N(m) and M(m) can be either one of the four 2'-O-methylated nucleosides. The relative amounts of the N(m) species are G = 45%, A = 25%, U = 20%, and C = 10%.
摘要
由海拉细胞核在体外合成的RNA含有多种封闭的甲基化5'-末端结构，这些结构可分为四种一般类型：(a) m(7)GppN(m)p...；(b) m(7)GppN(m)pM(m)p...；(c) m(7)GpppN(m)p...；以及(d) m(7)GpppN(m)pM(m)...。N(m)和M(m)都可以是四种2'-O-甲基化核苷中的任何一种。N(m)种类的相对含量为G = 45%，A = 25%，U = 20%，C = 10%。
相似文献
1
Synthesis of RNA containing a methylated blocked 5' terminus by HeLa nuclear homogenates.利用海拉细胞核匀浆合成含甲基化封闭5'端的RNA 。
Proc Natl Acad Sci U S A. 1975 Aug;72(8):2930-4. doi: 10.1073/pnas.72.8.2930.
2
Methylated, blocked 5 termini in HeLa cell mRNA.海拉细胞信使核糖核酸中甲基化、封闭的5'末端
Proc Natl Acad Sci U S A. 1975 May;72(5):1904-8. doi: 10.1073/pnas.72.5.1904.
3
Methylation and capping of RNA polymerase II primary transcripts by HeLa nuclear homogenates.HeLa细胞核匀浆对RNA聚合酶II初级转录本的甲基化和加帽作用。
Biochemistry. 1978 Mar 21;17(6):977-82. doi: 10.1021/bi00599a005.
4
Subcellular location of precursors to small nuclear RNA species C and D and of newly synthesized 5 S RNA in HeLa cells.HeLa细胞中小核RNA C和D种类的前体以及新合成的5S RNA的亚细胞定位。
Biochem Biophys Res Commun. 1978 Apr 14;81(3):915-9. doi: 10.1016/0006-291x(78)91438-9.
5
The effect of acridines on the synthesis of RNA in isolated nuclei and intact HeLa cells.吖啶对分离细胞核及完整海拉细胞中RNA合成的影响。
Experientia. 1970 Apr 15;26(4):364-5. doi: 10.1007/BF01896889.
6
The turnover of nuclear DNA-like RNA in HeLa cells.海拉细胞中类核DNA的核糖核酸周转
J Cell Biol. 1968 Oct;39(1):112-8. doi: 10.1083/jcb.39.1.112.
7
Messenger and heterogeneous nuclear RNA in HeLa cells: differential inhibition by cordycepin.HeLa细胞中的信使核糖核酸和不均一核核糖核酸：虫草素的差异抑制作用
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1878-85. doi: 10.1073/pnas.67.4.1878.
8
The methylation of adenovirus-specific nuclear and cytoplasmic RNA.腺病毒特异性核RNA和胞质RNA的甲基化
Nucleic Acids Res. 1976 Mar;3(3):749-65. doi: 10.1093/nar/3.3.749.
9
RNA synthesis in isolated HeLa cell nuclei.
Biochim Biophys Acta. 1976 Jan 5;418(1):29-38. doi: 10.1016/0005-2787(76)90324-5.
10
Reinitiation of synthesis of small cytoplasmic RNA species K and L in isolated HeLa cell nuclei in vitro.体外分离的HeLa细胞核中小细胞质RNA种类K和L合成的重新起始
Nucleic Acids Res. 1980 Jan 25;8(2):225-34. doi: 10.1093/nar/8.2.225.
引用本文的文献
1
Intracellular DNA of the parvovirus minute virus of mice is organized in a minichromosome structure.小鼠细小病毒的细胞内DNA以微型染色体结构存在。
J Virol. 1982 Mar;41(3):1044-54. doi: 10.1128/JVI.41.3.1044-1054.1982.
2
Specific in vitro initiation of transcription of simian virus 40 early and late genes occurs at the various cap nucleotides including cytidine.猿猴病毒40早期和晚期基因转录的特异性体外起始发生在包括胞苷在内的各种帽核苷酸处。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2174-8. doi: 10.1073/pnas.78.4.2174.
3
Initiation of transcription by RNA polymerase II in permeable, SV40-infected or noninfected, CVI cells; evidence for multiple promoters of SV40 late transcription.RNA聚合酶II在可渗透的、感染或未感染SV40的CVI细胞中启动转录；SV40晚期转录多个启动子的证据。
Nucleic Acids Res. 1981 Jan 24;9(2):215-36. doi: 10.1093/nar/9.2.215.
4
Characterization of the 5'-terminal cap structures of early simian virus 40 mRNA.早期猿猴病毒40信使核糖核酸5′末端帽结构的特性分析
J Virol. 1980 Sep;35(3):955-61. doi: 10.1128/JVI.35.3.955-961.1980.
5
5'-terminal cap structure in eucaryotic messenger ribonucleic acids.真核生物信使核糖核酸中的5'-末端帽结构。
Microbiol Rev. 1980 Jun;44(2):175-205. doi: 10.1128/mr.44.2.175-205.1980.
6
A human IFN-beta 1 gene deleted of promoter sequences upstream from the TATA box is controlled post-transcriptionally by dsRNA.一个缺失了TATA框上游启动子序列的人IFN-β1基因在转录后受双链RNA调控。
Nucleic Acids Res. 1984 Sep 25;12(18):6979-93. doi: 10.1093/nar/12.18.6979.
7
Minimal methylation of yeast messenger RNA.酵母信使核糖核酸的最小甲基化作用
Mol Biol Rep. 1976 Sep;3(1):9-17. doi: 10.1007/BF00357204.
8
Mercurated nucleotides: assessment of a new tool to study RNA synthesis and processing in isolated nuclei.汞化核苷酸：用于研究分离细胞核中RNA合成与加工的新工具的评估
Nucleic Acids Res. 1977 Sep;4(9):3109-22. doi: 10.1093/nar/4.9.3109.
9
Absence of detectable capping and methylating enzymes in influenza virions.流感病毒粒子中未检测到帽化和甲基化酶。
J Virol. 1978 Oct;28(1):75-83. doi: 10.1128/JVI.28.1.75-83.1978.
10
Mercurated nucleotides: assessment of a new tool to study RNA synthesis and processing in isolated nuclei.汞化核苷酸：用于研究分离细胞核中RNA合成与加工的新工具的评估
Nucleic Acids Res. 1977 Dec;4(12):4465-79. doi: 10.1093/nar/4.12.4465.
本文引用的文献
1
Sequences containing methylated nucleotides at the 5' termini of messenger RNAs: possible implications for processing.信使核糖核酸5'末端含有甲基化核苷酸的序列：对加工过程的潜在影响。
Cell. 1974 Nov;3(3):197-9. doi: 10.1016/0092-8674(74)90131-7.
2
Primary sequence of U-1 nuclear ribonucleic acid of Novikoff hepatoma ascites cells.诺维科夫肝癌腹水细胞U-1核糖核酸的一级序列。
J Biol Chem. 1974 Oct 25;249(20):6486-94.
3
The in vitro synthesis of avian myeloblastosis viral RNA sequences.禽成髓细胞瘤病毒RNA序列的体外合成
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3045-9. doi: 10.1073/pnas.71.8.3045.
4
The methylated constituents of L cell messenger RNA: evidence for an unusual cluster at the 5' terminus.L细胞信使核糖核酸的甲基化成分：5'末端存在异常簇集的证据。
Cell. 1975 Apr;4(4):387-94. doi: 10.1016/0092-8674(75)90159-2.
5
Units of transcription and translation: the relationship between heterogeneous nuclear RNA and messenger RNA.转录和翻译单位：核不均一RNA与信使RNA之间的关系
Cell. 1975 Jan;4(1):11-20. doi: 10.1016/0092-8674(75)90128-2.
6
Methylated, blocked 5 termini in HeLa cell mRNA.海拉细胞信使核糖核酸中甲基化、封闭的5'末端
Proc Natl Acad Sci U S A. 1975 May;72(5):1904-8. doi: 10.1073/pnas.72.5.1904.
7
Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-.呼肠孤病毒信使核糖核酸含有一个甲基化的、封闭的5'-末端结构：m-7G(5')ppp(5')G-MpCp- 。
Proc Natl Acad Sci U S A. 1975 Jan;72(1):362-6. doi: 10.1073/pnas.72.1.362.
8
A rapid assay technique for RNA ribose methylases.一种用于RNA核糖甲基化酶的快速检测技术。
Nucleic Acids Res. 1975 Apr;2(4):603-11. doi: 10.1093/nar/2.4.603.
9
Methylated simian virus 40-specific RNA from nuclei and cytoplasm of infected BSC-1 cells.来自受感染的BSC-1细胞核和细胞质的甲基化猿猴病毒40特异性RNA。
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2012-6. doi: 10.1073/pnas.72.6.2012.
10
Methylation-dependent translation of viral messenger RNAs in vitro.病毒信使核糖核酸在体外的甲基化依赖性翻译
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1189-93. doi: 10.1073/pnas.72.3.1189.
Zotero 插件