Groner Y, Hurwitz J
Proc Natl Acad Sci U S A. 1975 Aug;72(8):2930-4. doi: 10.1073/pnas.72.8.2930.
RNA synthesized in vitro by HeLa nuclei contains a variety of blocked methylated 5'-terminal structures that can be grouped into four general types: (a) m(7)GppN(m)p...; (b) m(7)GppN(m)pM(m)p...; (c) m(7)GpppN(m)p...; and (d) m(7)GpppN(m)pM(m).... Both N(m) and M(m) can be either one of the four 2'-O-methylated nucleosides. The relative amounts of the N(m) species are G = 45%, A = 25%, U = 20%, and C = 10%.
由海拉细胞核在体外合成的RNA含有多种封闭的甲基化5'-末端结构,这些结构可分为四种一般类型:(a) m(7)GppN(m)p...;(b) m(7)GppN(m)pM(m)p...;(c) m(7)GpppN(m)p...;以及(d) m(7)GpppN(m)pM(m)...。N(m)和M(m)都可以是四种2'-O-甲基化核苷中的任何一种。N(m)种类的相对含量为G = 45%,A = 25%,U = 20%,C = 10%。