Fukushima Restoration Support Headquarters, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan.
J Dermatol Sci. 2013 Jul;71(1):45-57. doi: 10.1016/j.jdermsci.2013.03.012. Epub 2013 Apr 11.
Although keratinocyte-derived factors are known to promote the proliferation and differentiation of human epidermal melanocytes, it is not fully understood whether fibroblast-derived factors work in a similar way.
The aim of this study is to clarify whether fibroblast-derived factors are involved in regulating the proliferation and differentiation of human melanocytes with or without keratinocytes using serum-free culture system.
Human epidermal melanoblasts and melanocytes were cultured in a serum-free growth medium supplemented with fibroblast-derived factors such as keratinocyte growth factor (KGF) with or without keratinocytes, and the effects of KGF on the proliferation and differentiation of melanocytes were studied.
KGF stimulated the proliferation of melanoblasts in the presence of dibutyryl cAMP (DBcAMP), basic fibroblast growth factor (bFGF), transferrin (Tf), and endothelin-1 (ET-1). Although KGF stimulated the differentiation, melanogenesis, and dendritogenesis in the presence of DBcAMP, Tf, and ET-1 without keratinocytes, KGF required the presence of keratinocytes for the stimulation of melanocyte proliferation.
These results suggest that fibroblast-derived KGF stimulates the proliferation of human melanoblasts in synergy with cAMP, bFGF, Tf, and ET-1, the differentiation of melanocytes in synergy with cAMP, Tf, and ET-1, and the proliferation of melanocytes in synergy with cAMP, Tf, ET-1, and undefined keratinocyte-derived factors.
虽然已知角质形成细胞衍生的因子可促进人类表皮黑素细胞的增殖和分化，但角质形成细胞衍生的因子是否以类似的方式作用于黑素细胞的增殖和分化还不完全清楚。
本研究旨在通过无血清培养系统，阐明有无角质形成细胞存在时，成纤维细胞衍生的因子（如角质细胞生长因子 [KGF]）是否参与调节人类黑素细胞的增殖和分化。
在无血清生长培养基中培养表皮黑素母细胞和黑素细胞，并添加角质形成细胞衍生的因子，如 KGF，并在有无角质形成细胞的情况下，研究 KGF 对黑素细胞增殖和分化的影响。
KGF 在二丁酰环磷酸腺苷（DBcAMP）、碱性成纤维细胞生长因子（bFGF）、转铁蛋白（Tf）和内皮素-1（ET-1）存在的情况下刺激黑素母细胞的增殖。虽然 KGF 在 DBcAMP、Tf 和 ET-1 存在的情况下无角质形成细胞存在时刺激黑素细胞的分化、黑色素生成和树突形成，但 KGF 刺激黑素细胞增殖需要角质形成细胞的存在。
这些结果表明，成纤维细胞衍生的 KGF 与 cAMP、bFGF、Tf 和 ET-1 协同刺激人类黑素母细胞的增殖，与 cAMP、Tf 和 ET-1 协同刺激黑素细胞的分化，与 cAMP、Tf、ET-1 和未定义的角质形成细胞衍生因子协同刺激黑素细胞的增殖。
