Feng Jiangfeng, Song Xiuzu, Zhang Beilei, Xiang Wenzhong
Hangzhou Third People's Hospital, Hangzhou, China.
Zhejiang Chinese Medical University, Hangzhou, China.
Lasers Med Sci. 2025 Jan 14;40(1):17. doi: 10.1007/s10103-025-04282-5.
Post-inflammatory hyperpigmentation (PIH) is a common cosmetic concern, often leading to significant psychological distress for the patients. With the widespread application of lasers including ablative fractional resurfacing (AFR) with a 10,600 nm CO laser, PIH caused by lasers is becoming increasingly common. But due to the absence of an appropriate animal research model, our understanding of pathophysiological mechanisms and preventive strategies for PIH remains limited.
This study aimed to establish an animal model to investigate PIH following AFR CO laser application, focusing on the dynamic changes in melanin, inflammatory cytokines, growth factors, and skin structures as PIH developed. We employed pigmented guinea pigs as our experimental subjects and conducted our research in two phases. In the first phase, we utilized three modes of AFR CO laser to identify which laser mode could induce PIH by monitoring dynamic melanin changes. In the second phase, the laser mode that most reliably induced PIH was applied to re-establish the PIH model. Pathophysiological changes during PIH progression were investigated through histopathological observations, real-time quantitative polymerase chain reaction, and two-photon microscopy.
We successfully established a replicable animal model for PIH following AFR CO laser application. We observed a significant increase in inflammatory cytokines and growth factors within the skin tissue by the second week, with stable pigmentation becoming apparent by the third week.
Our research provides a promising animal model for understanding and further investigating the mechanisms of PIH after laser procedures.
V (animal study).
炎症后色素沉着（PIH）是一个常见的美容问题，常常给患者带来严重的心理困扰。随着包括10600纳米CO2激光非剥脱性分次激光换肤（AFR）在内的激光广泛应用，激光引起的PIH越来越常见。但由于缺乏合适的动物研究模型，我们对PIH病理生理机制和预防策略的了解仍然有限。
本研究旨在建立一个动物模型，以研究AFR CO2激光应用后的PIH，重点关注PIH发生过程中黑色素、炎性细胞因子、生长因子和皮肤结构的动态变化。我们采用有色豚鼠作为实验对象，并分两个阶段进行研究。在第一阶段，我们利用三种AFR CO2激光模式，通过监测黑色素动态变化来确定哪种激光模式可诱导PIH。在第二阶段，应用最可靠诱导PIH的激光模式重建PIH模型。通过组织病理学观察、实时定量聚合酶链反应和双光子显微镜研究PIH进展过程中的病理生理变化。
我们成功建立了一个AFR CO2激光应用后可重复的PIH动物模型。我们观察到，到第二周时皮肤组织中的炎性细胞因子和生长因子显著增加，到第三周时色素沉着稳定且明显。
我们的研究为理解和进一步研究激光治疗后PIH的机制提供了一个有前景的动物模型。
V（动物研究）。
