Tambong James T, Xu Renlin, Bromfield Eden S P
Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, Ontario K1A 0C6, Canada.
Int J Syst Evol Microbiol. 2017 Apr;67(4):889-895. doi: 10.1099/ijsem.0.001698. Epub 2017 May 5.
The bacterial strain 2-92T, isolated from a field plot under long-term (>40 years) mineral fertilization, exhibited in vitro antagonistic properties against fungal pathogens. A polyphasic approach was undertaken to verify its taxonomic status. Strain 2-92T was Gram-reaction-negative, aerobic, non-spore-forming, motile by one or more flagella, and oxidase-, catalase- and urease-positive. The optimal growth temperature of strain 2-92T was 30 °C. 16S rRNA gene sequence analysis demonstrated that the strain is related to species of the genus Pseudomonas. Phylogenetic analysis of six housekeeping genes (dnaA, gyrB, recA, recF, rpoB and rpoD) revealed that strain 2-92T clustered as a distinct and well separated lineage with Pseudomonassimiae as the most closely related species. Polar lipid and fatty acid compositions corroborated the taxonomic position of strain 2-92T in the genus Pseudomonas. Phenotypic characteristics from carbon utilization tests could be used to differentiate strain 2-92T from closely related species of the genus Pseudomonas. DNA-DNA hybridization values (wet laboratory and genome-based) and average nucleotide identity data confirmed that this strain represents a novel species. On the basis of phenotypic and genotypic characteristics, it is concluded that this strain represents a separate novel species for which the name Pseudomonas canadensis sp. nov. is proposed, with type strain 2-92T (=LMG 28499T=DOAB 798T). The DNA G+C content is 60.30 mol%.
从长期（>40年）施用矿物肥料的田间地块分离出的菌株2-92T，在体外对真菌病原体具有拮抗特性。采用多相分类法来验证其分类地位。菌株2-92T革兰氏染色阴性，好氧，不产芽孢，通过一根或多根鞭毛运动，氧化酶、过氧化氢酶和脲酶呈阳性。菌株2-92T的最适生长温度为30°C。16S rRNA基因序列分析表明该菌株与假单胞菌属的物种相关。对六个管家基因（dnaA、gyrB、recA、recF、rpoB和rpoD）的系统发育分析显示，菌株2-92T聚为一个独特且分离良好的谱系，与最密切相关的物种类产假单胞菌聚在一起。极性脂质和脂肪酸组成证实了菌株2-92T在假单胞菌属中的分类地位。碳源利用试验的表型特征可用于区分菌株2-92T与假单胞菌属的近缘物种。DNA-DNA杂交值（湿实验室法和基于基因组法）以及平均核苷酸同一性数据证实该菌株代表一个新物种。基于表型和基因型特征，得出结论：该菌株代表一个单独的新物种，为此提出名为加拿大假单胞菌（Pseudomonas canadensis sp. nov.）的新名称，模式菌株为2-92T（=LMG 28499T=DOAB 798T）。DNA的G+C含量为60.30 mol%。
