生物膜培养基导致生物活性表面上磷灰石的形成。
Biofilm medium leads to apatite formation on bioactive surfaces.
机构信息
Department of Periodontology, Institute of Dentistry, University of Turku, Turku - Finland and Turku Clinical Biomaterials Centre - TCBC, University of Turku, Turku - Finland.
出版信息
J Appl Biomater Funct Mater. 2013 Sep 13;11(2):e95-8. doi: 10.5301/JABFM.5000154.
PURPOSE
When investigating apatite formation on biomaterial surfaces, simulated body fluid (SBF) is used as an in vitro solution, however, it does not provide an appropriate environment for the growth of bacterial biofilm. The aim of the present study was to compare the bioactivity in terms of apatite formation on two bioactive glass (BAG) composite surfaces using both SBF and bacterial-biofilm growing medium (BM).
METHODS
Polymer composite substrates with different percentages of BAG-particles (50% and 75% by weight) were prepared. Plain resin substrates were used as a negative control. The substrates were immersed in SBF and BM for 3 days. The surface and, subsequently, the cross-sections of the substrates were examined with scanning electron microscopy (SEM) and x-ray energy dispersive spectroscopy (EDS).
RESULTS
All the investigated BAG-composite surfaces showed apatite formation after immersion in SBF and BM liquid media.
CONCLUSIONS
The use of BM is a promising method for studies involving simultaneous biofilm growth and apatite formation on bioactive surfaces.
目的
在研究生物材料表面磷灰石形成时，通常使用模拟体液（SBF）作为体外溶液，但它不能为细菌生物膜的生长提供合适的环境。本研究的目的是比较两种生物活性玻璃（BAG）复合表面在使用 SBF 和细菌生物膜生长培养基（BM）时的成骨活性。
方法
制备了不同百分比 BAG 颗粒（重量比 50%和 75%）的聚合物复合基底。普通树脂基底用作阴性对照。将基底浸入 SBF 和 BM 中 3 天。用扫描电子显微镜（SEM）和 X 射线能量色散光谱（EDS）检查基底的表面和随后的横截面。
结果
所有研究的 BAG 复合材料表面在浸入 SBF 和 BM 液体介质后均显示出磷灰石形成。
结论
使用 BM 是一种很有前途的方法，可用于同时研究生物活性表面上的生物膜生长和磷灰石形成。
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