Ye Zhiqiang, Zhang Run, Song Bo, Dai Zhichao, Jin Dayong, Goldys Ewa M, Yuan Jingli
State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian 116024, China.
Dalton Trans. 2014 Jun 14;43(22):8414-20. doi: 10.1039/c4dt00179f.
A functional ruthenium(ii) complex, Ru(bpy)2(AN-bpy)2 (bpy: 2,2'-bipyridine, AN-bpy: 4-methyl-4'-(4-amino-3-nitro-phenoxy-methylene)-2,2'-bipyridine), has been designed and synthesized as a turn-on luminescent probe for the imaging of hypochlorous acid (HOCl) in living cells. Due to the intramolecular photoinduced electron transfer (PET), the ruthenium(ii) complex itself is almost non-luminescent. However, it can specifically and rapidly react with HOCl in aqueous media to afford a highly luminescent derivative, Ru(bpy)2(HM-bpy)2 (HM-bpy: 4-hydroxymethyl-4'-methyl-2,2'-bipyridine), accompanied by a 110-fold luminescence enhancement. Taking advantage of high specificity and sensitivity, and excellent photophysical properties of the ruthenium(ii) complex probe, Ru(bpy)2(AN-bpy)2 was successfully applied to the luminescence imaging of the exogenous HOCl in living HeLa cells and the endogenous HOCl in porcine neutrophils. The results corroborate that indeed Ru(bpy)2(AN-bpy)2 is a useful luminescent probe for the monitoring of HOCl in biological systems.
一种功能性钌（II）配合物Ru(bpy)2(AN-bpy)2（bpy：2,2'-联吡啶，AN-bpy：4-甲基-4'-(4-氨基-3-硝基苯氧基亚甲基)-2,2'-联吡啶）已被设计并合成，作为一种用于活细胞中次氯酸（HOCl）成像的开启型发光探针。由于分子内光诱导电子转移（PET），钌（II）配合物本身几乎不发光。然而，它能在水性介质中与HOCl特异性快速反应，生成一种高发光衍生物Ru(bpy)2(HM-bpy)2（HM-bpy：4-羟甲基-4'-甲基-2,2'-联吡啶），伴随着110倍的发光增强。利用钌（II）配合物探针的高特异性、高灵敏度以及优异的光物理性质，Ru(bpy)2(AN-bpy)2成功应用于活HeLa细胞中外源HOCl和猪中性粒细胞中内源性HOCl的发光成像。结果证实，Ru(bpy)2(AN-bpy)2确实是一种用于监测生物体系中HOCl的有用发光探针。
