喉癌中的全球 DNA 甲基化状态。
Global DNA methylation status in laryngeal cancer.
机构信息
Department of Genetics, Wroclaw Medical University, Wroclaw, Poland.
出版信息
Head Neck. 2014 Mar;36(3):419-24. doi: 10.1002/hed.23315. Epub 2013 Jun 1.
BACKGROUND
The purpose of our study was to evaluate if DNA methylation level in leukocytes may be used as a surrogate marker of genome methylation status in laryngeal cancer tissues.
METHODS
We evaluated global DNA methylation using an ultra performance liquid chromatography (UPLC)-based method to assess the total content of 5-methylcytosine (5 mC).
RESULTS
The study was performed on DNA isolated from cancer tissues, adjacent normal tissues, and peripheral blood leukocytes in a group of 72 patients with laryngeal cancer. DNA hypomethylation was found in tumor tissue (56%) and normal tissue (49%). There was a significant correlation between the levels of 5 mC in these 2 types of tissue. There was no significant DNA hypomethylation in blood. A negative correlation between tumor grade and blood levels of 5 mC was found.
CONCLUSION
The level of leukocyte DNA methylation measured using total 5 mC content cannot be used as a surrogate marker for genome methylation status in laryngeal cancer tissues.
背景
本研究旨在评估白细胞中的 DNA 甲基化水平是否可用作喉癌组织中基因组甲基化状态的替代标志物。
方法
我们使用超高效液相色谱 (UPLC) 法评估了全基因组 DNA 甲基化水平，以评估 5-甲基胞嘧啶 (5 mC) 的总含量。
结果
本研究在一组 72 例喉癌患者的癌组织、相邻正常组织和外周血白细胞中进行。在肿瘤组织（56%）和正常组织（49%）中发现 DNA 低甲基化。这两种组织中 5 mC 水平之间存在显著相关性。在血液中没有明显的 DNA 低甲基化。在肿瘤分级与血液中 5 mC 水平之间发现了负相关。
结论
使用总 5 mC 含量测量的白细胞 DNA 甲基化水平不能用作喉癌组织中基因组甲基化状态的替代标志物。
Zotero 插件