Zhu Guochen, Lou Weihua
Department of Otolaryngology, Wuxi Second People's Hospital, Affiliated with Nanjing Medical University, Wuxi, Jiangsu, China.
Head Neck. 2014 Apr;36(4):481-6. doi: 10.1002/hed.23321. Epub 2013 Jun 1.
Because of ease of harvest and low immunogenicity, xenogeneic acellular nerve graft (XANG) may be an alternative to autologous nerve to repair facial nerve defects.
Facial nerve defects of Wistar rats were repaired by XANG, and nerve gap regeneration was investigated by electrophysiological test, horseradish peroxidase (HRP) retrograde tracing and histomorphometric analysis, as compared to autograft.
Twenty weeks after the grafting, electrophysiology showed that whisker pad muscles responded to the electrical stimuli given at the site proximal to the transplantation in 2 groups. Some HRP-labeled facial motorneurons were located on the facial nucleus of the operated side, and an abundance of myelinated axons were found at the middle of the grafts and obvious motor endplates in the target muscles in 2 groups, although they were inferior to the contralateral side in numbers.
XANG represents an alternative approach for the reconstruction of peripheral facial nerve defects.
由于易于获取且免疫原性低，异种脱细胞神经移植物（XANG）可能是修复面神经缺损的自体神经替代物。
用XANG修复Wistar大鼠的面神经缺损，并通过电生理测试、辣根过氧化物酶（HRP）逆行追踪和组织形态计量分析研究神经间隙再生情况，与自体移植进行比较。
移植20周后，电生理显示两组中触须垫肌肉对移植部位近端给予的电刺激有反应。两组中一些HRP标记的面神经运动神经元位于手术侧的面神经核上，在移植物中部发现大量有髓轴突，在靶肌肉中发现明显的运动终板，尽管数量上不如对侧。
XANG是重建周围面神经缺损的一种替代方法。
