Adachi Y, Katoh H, Fuchi I, Yamamoto T
Second Department of Internal Medicine, Kinki University School of Medicine, Osaka, Japan.
Clin Biochem. 1990 Jun;23(3):247-51. doi: 10.1016/0009-9120(90)90705-y.
Serum bilirubin fractions were determined by a newly developed, high performance liquid chromatography method in 23 healthy subjects, and in 15 patients with Gilbert's syndrome, five with type 2 Crigler-Najjar syndrome, seven with hemolytic disorders, and 26 with neonatal jaundice. In the healthy subjects, 92.6% of the bilirubin was unconjugated (UCB), 6.2% was bilirubin monoglucoronide (BMG), and 0.5% was bilirubin diglucuronide (BDG). Delta bilirubin (B delta) was not detected. In the patients, the percentage of UCB was significantly higher and that of BMG was significantly lower than in the healthy subjects. The proportion of BDG tended to decrease in Gilbert's and type 2 Crigler-Najjar syndromes; the proportion of B delta tended to increase in hyperbilirubinemia, except in Gilbert's syndrome. In particular, B delta was frequently detected in serum which also contained BDG (mainly in hemolytic disorders) or which presented with high concentrations (above 100 mumol/L) of UCB (mainly in type 2 Crigler-Najjar syndrome and neonatal jaundice). Trace amounts of (Z,E)- and/or (E,Z)-UCB were detected in approximately one fourth of the serum samples analyzed.
采用新开发的高效液相色谱法测定了23名健康受试者、15名吉尔伯特综合征患者、5名2型克里格勒-纳贾尔综合征患者、7名溶血性疾病患者和26名新生儿黄疸患者的血清胆红素组分。在健康受试者中，92.6%的胆红素为未结合胆红素（UCB），6.2%为胆红素单葡萄糖醛酸酯（BMG），0.5%为胆红素双葡萄糖醛酸酯（BDG）。未检测到δ胆红素（Bδ）。在患者中，UCB的百分比显著高于健康受试者，而BMG的百分比显著低于健康受试者。在吉尔伯特综合征和2型克里格勒-纳贾尔综合征中，BDG的比例趋于降低；在高胆红素血症中，Bδ的比例趋于增加，但吉尔伯特综合征除外。特别是，在同时含有BDG的血清（主要在溶血性疾病中）或UCB浓度较高（高于100μmol/L）的血清（主要在2型克里格勒-纳贾尔综合征和新生儿黄疸中）中经常检测到Bδ。在大约四分之一的分析血清样本中检测到痕量的（Z,E）-和/或（E,Z）-UCB。
