利用变性梯度凝胶电泳（DGGE）分析人参土壤中的细菌群落。
Analysis of Bacterial Community in the Ginseng Soil Using Denaturing Gradient Gel Electrophoresis (DGGE).
机构信息
Agricultural College & Research Institute, Tamil Nadu Agricultural University, Tiruchirappalli, 620 009 Tamil Nadu India.
出版信息
Indian J Microbiol. 2012 Jun;52(2):286-8. doi: 10.1007/s12088-011-0193-3. Epub 2011 Jul 3.
Abstract
The objective of this work was to determine the shifts in the PCR-DGGE profiles of bacterial communities associated with the rhizosphere soil of ginseng at varying age levels. Differences in the dominance of intense DNA bands in the DGGE profile was observed over the age of the plants indicating the fluctuation in the microbial community structure. The bacterial orders of actinomycetales of Actinobacteria and Spingomonadales and Rhizobiales of α-Proteobacteria were predominant in the ginseng soil.
摘要
本研究旨在确定不同年龄阶段人参根际土壤细菌群落 PCR-DGGE 图谱的变化。DGGE 图谱中强 DNA 带的优势程度随植物年龄的不同而变化，表明微生物群落结构的波动。人参土壤中优势的细菌类群为放线菌目的放线菌和单胞菌目的α-变形菌以及根瘤菌目的α-变形菌。
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