Pandey Santwana, Raza Shahid, Bhatta Chandra Prakash
Lecturer, Department of Microbiology, Kantipur Dental College & Teaching Hospital , Kathmandu, Nepal .
J Clin Diagn Res. 2013 Apr;7(4):638-41. doi: 10.7860/JCDR/2013/4752.2871. Epub 2013 Apr 1.
Bloodstream infections are associated with a significant patient morbidity and mortality. The detection of microorganisms in the patients' blood has a great diagnostic and prognostic significance. The early positive results provide valuable diagnostic information, based on which the appropriate antimicrobial therapy can be initiated.
To know the aetiology of the bloodstream infections in the Kathmandu Medical College, Nepal and the antibiotic sensitivity patterns of the causative organisms.
The blood specimens which were received from May 2010 to October 2010 in Kathmandu Medical College and Teaching Hospital, Kathmandu, Nepal, were processed and all the positive isolates were included in the study. The isolates were identified by the standard laboratory procedures. The antibiotic susceptibility patterns were determined by the modified Kirby Bauer antibiotic sensitivity method.
Of the 1089 blood cultures which were received with the suspected cases of blood stream infections, 138 (12.6 %) were bacteriologically positive. Salmonella serotypes were isolated in 42.7% cases of blood stream infections, followed by Klebsiella pneumoniae in 19.5%, Staphylococcus aureus in 15.9% and others in the rest of the cases. All the gram-negative bacilli isolates showed lower degrees of resistance to amikacin and ofloxacin. All the gram positive isolates were sensitive to amikacin, oxacillin and vancomycin.
This study stresses on the need for a continued screening and surveillance in the routine blood culture technique for starting with the empiric therapy for blood borne infections.
血流感染与患者的高发病率和死亡率相关。检测患者血液中的微生物具有重要的诊断和预后意义。早期的阳性结果可提供有价值的诊断信息，据此可启动适当的抗菌治疗。
了解尼泊尔加德满都医学院血流感染的病因及病原菌的抗生素敏感性模式。
对2010年5月至2010年10月在尼泊尔加德满都加德满都医学院及教学医院采集的血液标本进行处理，所有阳性分离株均纳入研究。采用标准实验室程序对分离株进行鉴定。采用改良的 Kirby Bauer 抗生素敏感性方法测定抗生素敏感性模式。
在1089份疑似血流感染病例的血培养标本中，138份（12.6%）细菌学检测呈阳性。血流感染病例中42.7%分离出沙门氏菌血清型，其次是肺炎克雷伯菌，占19.5%，金黄色葡萄球菌占15.9%，其余病例分离出其他病原菌。所有革兰氏阴性杆菌分离株对阿米卡星和氧氟沙星的耐药程度较低。所有革兰氏阳性分离株对阿米卡星、苯唑西林和万古霉素敏感。
本研究强调在常规血培养技术中持续进行筛查和监测对于开始针对血源性感染进行经验性治疗的必要性。
