Varma S D, Devamanoharan P S, Morris S M
University of Maryland, School of Medicine, Department of Ophthalmology, Baltimore 21201.
Exp Eye Res. 1990 Jun;50(6):805-12. doi: 10.1016/0014-4835(90)90131-d.
Intact rat lenses were incubated in riboflavin-containing Tyrode solution or medium-199, generating photochemically active species of oxygen and the oxidative stress measured in terms of the decrease in active accumulation of rubidium, and the fall in the levels of glutathione and ATP. Addition of pyruvate to the medium prevented the tissue against oxidative damage as evidenced by a greater accumulation of rubidium and higher levels of glutathione and ATP. Pyruvate was thus found to be effective against the toxicity of oxygen derivatives, particularly the hydrogen peroxide. In dark experiments also, conducted in glucose-free medium, the uptake of rubidium was substantially greater in the presence of pyruvate. The levels of ATP were also higher. These results, therefore, suggest that this ketoacid is beneficial to the tissue through its ability to decompose H2O2 as well through providing a metabolic support. The development of in vitro cataract under the photochemical effects of riboflavin and oxygen was also effectively thwarted by pyruvate. The results are thus potentially useful from the point of view of developing pyruvate and similar compounds as effective anticataract agents.
将完整的大鼠晶状体置于含核黄素的台氏液或199培养基中孵育，产生光化学活性氧物质，并根据铷的活性积累减少、谷胱甘肽和三磷酸腺苷水平下降来测定氧化应激。向培养基中添加丙酮酸可防止组织受到氧化损伤，这表现为铷的积累增加以及谷胱甘肽和三磷酸腺苷水平升高。因此发现丙酮酸对氧衍生物的毒性有效，尤其是对过氧化氢。在无葡萄糖培养基中进行的黑暗实验中，存在丙酮酸时铷的摄取量也显著增加。三磷酸腺苷水平也更高。因此，这些结果表明，这种酮酸通过其分解过氧化氢的能力以及提供代谢支持对组织有益。丙酮酸也有效地阻止了在核黄素和氧的光化学作用下体外白内障的形成。因此，从开发丙酮酸及类似化合物作为有效的抗白内障药物的角度来看，这些结果可能具有实用价值。
