Lütjen-Drecoll E, Schenholm M, Tamm E, Tengblad A
Department of Anatomy, University of Erlangen-Nürnberg, F.R.G.
Exp Eye Res. 1990 Jul;51(1):55-63. doi: 10.1016/0014-4835(90)90170-y.
Hyaluronic acid was visualized using a new histochemical technique: the hyaluronic acid-binding region of the cartilage proteoglycan was isolated, linked with biotin and used in histological sections as a ligand for hyaluronic acid. Staining was performed using the avidin-biotin-peroxidase technique. The presence of hyaluronic acid in the anterior segments of rabbit and monkey eyes was studied in fresh-frozen, as well as in fixed paraffin sections; addition of cetyl-pyridinium chloride to the fixative was essential. In both species intense staining was seen in the stroma of the conjunctiva, the connective tissue of the ciliary processes, and on the apical membranes of the corneal endothelium. Species differences were observed in both conventional and unconventional outflow pathways. In rabbits, the tissue located directly adjacent to the aqueous plexus and the connective tissue surrounding the poorly developed ciliary muscle were stained. In monkeys, only the anterior, non-filtering part of the trabecular meshwork and the outer wall of Schlemm's canal showed intense staining in paraffin sections. In frozen sections, the inner wall (cribriform region of the trabecular meshwork) was also stained. The spaces between the anterior part of the ciliary muscle bundles remained almost unstained.
分离软骨蛋白聚糖的透明质酸结合区域，与生物素连接，并在组织切片中用作透明质酸的配体。使用抗生物素蛋白-生物素-过氧化物酶技术进行染色。研究了兔和猴眼前节新鲜冷冻以及固定石蜡切片中透明质酸的存在情况；向固定剂中添加十六烷基氯化吡啶至关重要。在这两个物种中，在结膜基质、睫状体的结缔组织以及角膜内皮的顶端膜上均可见强烈染色。在传统和非传统流出途径中均观察到物种差异。在兔子中，直接毗邻房水丛的组织以及发育不良的睫状肌周围的结缔组织被染色。在猴子中，在石蜡切片中，仅小梁网的前部非滤过部分和施莱姆管的外壁显示强烈染色。在冷冻切片中，内壁（小梁网的筛状区域）也被染色。睫状肌束前部之间的间隙几乎未染色。
