Barbiéri C L, Doine A I, Freymuller E
Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, São Paulo, Brasil.
Exp Parasitol. 1990 Aug;71(2):218-28. doi: 10.1016/0014-4894(90)90024-7.
Analysis of lysosomes through acid phosphatase cytochemistry at the electron microscopy level has been performed in spleen and foot lesions from Leishmania-infected hamsters. The results showed that there is lysosomal depletion in macrophages from Leishmania donovani chagasi-infected hamster spleen and similar findings were obtained from foot lesions of Leishmania mexicana amazonensis-infected hamsters. The distribution of acid phosphatase and thiamine pyrophosphatase was also examined in the Golgi apparatus. It was possible to demonstrate that the activity of ACP is absent in infected macrophages from spleen and foot lesions of Leishmania-infected hamster while the distribution of TPP was very similar in control and infected macrophages from both systems. These results provide evidence that the lysosomal depletion can occur at the ACP synthesis and/or glycosylation level.
通过电子显微镜水平的酸性磷酸酶细胞化学对利什曼原虫感染的仓鼠脾脏和足部病变中的溶酶体进行了分析。结果表明，来自杜氏利什曼原虫恰加斯亚种感染的仓鼠脾脏巨噬细胞中存在溶酶体耗竭现象，并且从墨西哥利什曼原虫亚马逊亚种感染的仓鼠足部病变中也获得了类似的结果。还在高尔基体中检测了酸性磷酸酶和硫胺素焦磷酸酶的分布。有可能证明，在利什曼原虫感染的仓鼠脾脏和足部病变的感染巨噬细胞中不存在酸性磷酸酶（ACP）的活性，而硫胺素焦磷酸（TPP）在两个系统的对照巨噬细胞和感染巨噬细胞中的分布非常相似。这些结果提供了证据，表明溶酶体耗竭可能发生在ACP合成和/或糖基化水平。
