Severson D W, Erickson E H, Williamson J L, Aiken J M
Department of Veterinary Science, University of Wisconsin, Madison 53706.
Experientia. 1990 Jul 15;46(7):737-9. doi: 10.1007/BF01939951.
We employed in vitro translation of mRNA and product separation using SDS-PAGE to examine the heat-shock response of the worker honey bee. Increases in the levels of 6 translatable RNA populations were observed following heat stress. The greatest response was observed among bees aged 9 days. Slight levels of induction of 70 and 82 kDa heat shock proteins were evident among bees taken directly from the colony.
我们采用mRNA的体外翻译和使用SDS-PAGE进行产物分离的方法,来检测工蜂的热休克反应。热应激后观察到6种可翻译RNA群体的水平有所增加。在9日龄的蜜蜂中观察到最大的反应。直接从蜂群中取出的蜜蜂中,70 kDa和82 kDa热休克蛋白有轻微的诱导水平。