Linking Histone Methylation States and Transcriptional Regulation in Thermo-Tolerant and Thermo-Susceptible L. Subspecies in Response to Heat Stress.

Genetic and epigenetic responses to environmental cues of worker honeybees mediate synthesis, a key mechanism to tolerate high ambient temperatures in In this study, the chromatin immunoprecipitation assay followed by qPCR were used to determine alterations in histone methylation states (H3K27me2, H3K27me3, H3K4me2, and H3K4me3) associated with / in (thermo-tolerant subspecies) and (thermo-susceptible subspecies) after heat treatment. The results revealed significant changes in enrichment folds of histone methylation states associated with /. Indeed, the enrichment of H3K27me2 decreased strongly in response to heat stress. Changes in histone methylation states were significantly higher in samples compared to samples. Our study provides a new perception on linking histone post-translational methylation as an epigenetic mechanism of gene regulation with in subspecies exposed to heat stress.