Zheng Fan, Xu Gang, Zhou Jia, Xie Changping, Cui Hongguang, Miao Weiguo, Kang Zhensheng, Zheng Li
College of Plant Protection, Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, Haikou, 570228, People's Republic of China.
State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, People's Republic of China.
Arch Virol. 2019 Aug;164(8):2209-2213. doi: 10.1007/s00705-019-04314-w. Epub 2019 Jun 3.
The complete genome of a double-stranded RNA (dsRNA) mycovirus, Phoma matteuccicola partitivirus 1 (PmPV1) was sequenced. It consists of two dsRNA segments, 1664 bp (dsRNA-1) and 1383 bp (dsRNA-2) in length, each containing a single open reading frame (ORF) potentially encoding a 46.78-kDa protein and a 40.92-kDa protein, respectively. dsRNA-1 encodes a putative polypeptide with a conserved RNA-dependent RNA polymerase (RdRp) domain that shows sequence similarity to the corresponding proteins of partitiviruses. The protein encoded by dsRNA-2 has no significant similarity to the typical coat proteins (CPs) of partitiviruses, but structure analysis nevertheless suggested that it might function as a coat protein. Purified viral particles of PmPV1 were isometric and approximately 29 nm in diameter. Phylogenetic analysis showed that PmPV1 is closely related to members of the genus Gammapartitivirus within the family Partitiviridae but forms a separate branch with Colletotrichum acutatum RNA virus 1 and Ustilaginoidea virens partitivirus 2. This is the first report of the full-length nucleotide sequence of a novel virus of the genus Gammapartitivirus infecting P. matteuccicola strain LG915, the causal agent of leaf blight of Curcuma wenyujin.
对一种双链RNA（dsRNA）真菌病毒——马蒂乌奇拟茎点霉分病毒1（PmPV1）的全基因组进行了测序。它由两个dsRNA片段组成，长度分别为1664 bp（dsRNA - 1）和1383 bp（dsRNA - 2），每个片段都包含一个单一的开放阅读框（ORF），分别可能编码一个46.78 kDa的蛋白质和一个40.92 kDa的蛋白质。dsRNA - 1编码一种具有保守的RNA依赖RNA聚合酶（RdRp）结构域的推定多肽，该结构域与分病毒的相应蛋白质具有序列相似性。dsRNA - 2编码的蛋白质与分病毒的典型衣壳蛋白（CP）没有显著相似性，但结构分析表明它可能起到衣壳蛋白的作用。纯化的PmPV1病毒粒子呈等轴状，直径约为29 nm。系统发育分析表明，PmPV1与分病毒科γ分病毒属的成员密切相关，但与尖孢炭疽菌RNA病毒1和稻曲病菌分病毒2形成一个单独的分支。这是关于一种感染温郁金叶枯病病原菌马蒂乌奇拟茎点霉LG915菌株的新型γ分病毒属病毒全长核苷酸序列的首次报道。
