Agricultural College; Shi He Zi University; Xinjiang, PR China.
Plant Signal Behav. 2013 Aug;8(8). doi: 10.4161/psb.24952. Epub 2013 May 15.
MicroRNAs (miRNAs) play important roles in plant growth and development and abiotic stress responses. We report here that heat stress rapidly induces miR398 and reduces transcript of its target gene CSD2. Transgenic plants overexpressing the miR398-resistant form of CSD2 are more sensitive to heat stress than transgenic plants overexpressing normal coding sequence of CSD2. Expression of heat stress transcription factors (HSFs) and heat shock proteins (HSPs) is reduced in the heat-sensitive transgenic plants overexpressing miR398-resistant form of CSD2. Our results suggest that downregulation of CSD2 by the heat-inducible miR398 is required for thermotolerance in Arabidopsis.
MicroRNAs (miRNAs) 在植物生长发育和非生物胁迫响应中发挥重要作用。我们在这里报告，热胁迫能快速诱导 miR398 的表达并降低其靶基因 CSD2 的转录本。过表达 miR398 抗性形式 CSD2 的转基因植物比过表达 CSD2 正常编码序列的转基因植物对热胁迫更为敏感。在过表达 miR398 抗性形式 CSD2 的热敏感转基因植物中，热应激转录因子 (HSFs) 和热休克蛋白 (HSPs) 的表达降低。我们的结果表明，CSD2 由热诱导的 miR398 下调是拟南芥耐热性所必需的。
