水稻 ORC 亚基间的相互作用。
Interactions among rice ORC subunits.
机构信息
The State Key Laboratory of Plant Physiology and Biochemistry; College of Life Sciences; Zhejiang University; Hangzhou, PR China.
出版信息
Plant Signal Behav. 2013 Aug;8(8). doi: 10.4161/psb.25007. Epub 2013 May 17.
The origin recognition complex (ORC) is composed of six subunits and plays an important role in DNA replication in all eukaryotes. The ORC subunits OsORC6 as well as the other five ORC subunits in rice were experimentally isolated and sequenced. It indicated that there also exist six ORC subunits in rice. Results of RT-PCR indicated that expression of all the rice ORC genes are no significant difference under 26°C and 34°C. Yeast two hybridization indicated that OsORC2, -3, -5 interact with each other. OsORC5 can then bind OsORC4 to form the OsORC2, -3,-4,-5 core complex. It suggested that the basic interactions have been conserved through evolution. No binding of OsORC1 and OsORC6 with the other subunits were observed. A model of ORC complex in rice is proposed.
起始识别复合物（ORC）由六个亚基组成，在所有真核生物的 DNA 复制中发挥重要作用。ORC 亚基 OsORC6 以及水稻中的其他五个 ORC 亚基已被实验分离和测序。这表明在水稻中也存在六个 ORC 亚基。RT-PCR 结果表明，在 26°C 和 34°C 下，所有水稻 ORC 基因的表达没有显著差异。酵母双杂交表明，OsORC2、-3、-5 相互作用。OsORC5 然后可以与 OsORC4 结合形成 OsORC2、-3、-4、-5 核心复合物。这表明基本相互作用在进化过程中得到了保守。没有观察到 OsORC1 和 OsORC6 与其他亚基的结合。提出了一个水稻 ORC 复合物的模型。
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