Brasil Juliana Nogueira, Costa Carinne N Monteiro, Cabral Luiz Mors, Ferreira Paulo C G, Hemerly Adriana S
Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Centro Universitário Christus, Fortaleza, CE, Brazil.
Genet Mol Biol. 2017;40(1 suppl 1):276-291. doi: 10.1590/1678-4685-GMB-2016-0118. Epub 2017 Mar 16.
The multiplication of cells in all living organisms requires a tight regulation of DNA replication. Several mechanisms take place to ensure that the DNA is replicated faithfully and just once per cell cycle in order to originate through mitoses two new daughter cells that contain exactly the same information from the previous one. A key control mechanism that occurs before cells enter S phase is the formation of a pre-replication complex (pre-RC) that is assembled at replication origins by the sequential association of the origin recognition complex, followed by Cdt1, Cdc6 and finally MCMs, licensing DNA to start replication. The identification of pre-RC members in all animal and plant species shows that this complex is conserved in eukaryotes and, more importantly, the differences between kingdoms might reflect their divergence in strategies on cell cycle regulation, as it must be integrated and adapted to the niche, ecosystem, and the organism peculiarities. Here, we provide an overview of the knowledge generated so far on the formation and the developmental controls of the pre-RC mechanism in plants, analyzing some particular aspects in comparison to other eukaryotes.
所有生物体内细胞的增殖都需要对DNA复制进行严格调控。为确保DNA能被精确复制且在每个细胞周期仅复制一次，从而通过有丝分裂产生两个包含与前一个细胞完全相同信息的新子细胞，会发生多种机制。在细胞进入S期之前发生的一种关键控制机制是形成前复制复合体（pre-RC），它通过起始识别复合体、随后是Cdt1、Cdc6以及最终的MCMs的顺序结合，在复制起点组装而成，从而许可DNA开始复制。在所有动植物物种中对前复制复合体成员的鉴定表明，这种复合体在真核生物中是保守的，更重要的是，不同生物界之间的差异可能反映了它们在细胞周期调控策略上的分歧，因为它必须与生态位、生态系统以及生物体特性相整合并适应。在此，我们概述了目前关于植物中前复制复合体机制的形成和发育控制所产生的知识，并与其他真核生物相比分析了一些特定方面。
