通过体内生物发光成像和死后多色组织学分析对小鼠中枢神经系统中干细胞移植进行定量评估。

Quantitative evaluation of stem cell grafting in the central nervous system of mice by in vivo bioluminescence imaging and postmortem multicolor histological analysis.

作者信息

Reekmans Kristien, De Vocht Nathalie, Praet Jelle, Le Blon Debbie, Hoornaert Chloé, Daans Jasmijn, Van der Linden Annemie, Berneman Zwi, Ponsaerts Peter

机构信息

Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Campus Drie Eiken (CDE-S6.51), Universiteitsplein 1, Antwerp (Wilrijk), 2610, Belgium.

出版信息

Methods Mol Biol. 2013;1052:125-41. doi: 10.1007/7651_2013_17.

DOI:10.1007/7651_2013_17

PMID:23733539

Abstract

Stem cell transplantation in the central nervous system (CNS) is currently under intensive investigation as a novel therapeutic approach for a variety of brain disorders and/or injuries. However, one of the main hurdles at the moment is the lack of standardized procedures to evaluate cell graft survival and behavior following transplantation into CNS tissue, thereby leading to the publication of confusing and/or conflicting research results. In this chapter, we therefore provide validated in vivo bioluminescence and postmortem histological procedures to quantitatively determine: (a) the survival of grafted stem cells, and (b) the microglial and astroglial cell responses following cell grafting.

摘要

作为治疗各种脑部疾病和/或损伤的一种新型治疗方法，中枢神经系统（CNS）中的干细胞移植目前正在深入研究中。然而，目前的主要障碍之一是缺乏标准化程序来评估移植到CNS组织后细胞移植的存活情况和行为，从而导致发表的研究结果令人困惑和/或相互矛盾。因此，在本章中，我们提供了经过验证的体内生物发光和死后组织学程序，以定量确定：（a）移植干细胞的存活情况，以及（b）细胞移植后小胶质细胞和星形胶质细胞的反应。