大鼠组蛋白基因H1fv、H1d、H1t、Th2a和Th2b的染色体定位

Chromosome mapping of rat histone genes H1fv, H1d, H1t, Th2a and Th2b.

作者信息

Walter L, Klinga-Levan K, Helou K, Albig W, Drabent B, Doenecke D, Günther E, Levan G

机构信息

Division of Immunogenetics, University of Göttingen, Germany.

出版信息

Cytogenet Cell Genet. 1996;75(2-3):136-9. doi: 10.1159/000134464.

DOI:10.1159/000134464

PMID:9040779

Abstract

Chromosome assignment of the rat histone genes H1t, H1d (H1.4), H1fv (H10), Th2a and Th2b is described. The testicularly expressed histone genes H1t, Th2a and Th2b could be assigned to rat chromosome (RNO) 17 by PCR analysis of somatic cell hybrid DNAs. The H1d gene was mapped to RNO17p12-->p11 by FISH. These genes might form a histone gene cluster homologous to that found on HSA6p21.3 in humans and MMU13A2-3 in mice. The rat histone H1fv gene was assigned to RNO7 by PCR. This result allows the inclusion of rat H1fv to an established conserved group of syntenic genes in rat, mouse and human on chromosomes RNO7, MMU15 and HSA22, respectively.

摘要

描述了大鼠组蛋白基因H1t、H1d（H1.4）、H1fv（H10）、Th2a和Th2b的染色体定位。通过对体细胞杂交DNA进行PCR分析，可将睾丸表达的组蛋白基因H1t、Th2a和Th2b定位到大鼠染色体（RNO）17上。通过荧光原位杂交（FISH）将H1d基因定位到RNO17p12→p11。这些基因可能形成一个与人类HSA6p21.3和小鼠MMU13A2 - 3上发现的组蛋白基因簇同源的基因簇。通过PCR将大鼠组蛋白H1fv基因定位到RNO7上。这一结果使得大鼠H1fv能够归入分别位于大鼠染色体RNO7、小鼠染色体MMU15和人类染色体HSA22上已确定的保守同线基因群中。