Department of Orthopaedics, Shanghai tenth People's Hospital, Tongji University School of Medicine, No,301 Middle Yanchang Road, Shanghai, 200072, China.
Cancer Cell Int. 2013 Jun 4;13(1):56. doi: 10.1186/1475-2867-13-56.
Nowadays it remains a controversial issue whether a correlation exists between the apoptosis rate of tumor tissue and the prognosis of the patients. We aimed to explore the prognostic significance of apoptosis index of human osteosarcoma tissue.
The technique of terminal DNA breakpoints in situ 3 - hydroxy end labeling (TUNEL) was used to detect and analysis apoptosis index in 56 osteosarcoma specimens. The relationships between apoptosis index of tumor tissue and long term survival of patients as well as pathologic classification, tumor clinical stages, tumor size and level of serum alkaline phosphatase were analyzed.
Our studies showed the cases with high apoptosis index had significantly longer survival time. Apoptosis index in osteosarcoma tissue was correlated with tumor size and level of serum alkaline phosphatase but not with pathologic classifications and clinical stages of tumor.
Our results demonstrated that apoptosis index of osteosarcoma tissue combined with serum alkaline phosphatase could used as valid indicators to predicate the malignant level and prognosis of osteosarcoma cases, which would contribute to enhance efficacy of clinical treatments for osteosarcoma.
目前，肿瘤组织细胞凋亡率与患者预后之间是否存在相关性仍然存在争议。本研究旨在探讨人骨肉瘤组织细胞凋亡指数的预后意义。
采用原位末端标记法（TUNEL）检测 56 例骨肉瘤标本中的细胞凋亡指数，并分析肿瘤组织细胞凋亡指数与患者长期生存及病理分级、肿瘤临床分期、肿瘤大小和血清碱性磷酸酶水平的关系。
研究表明，细胞凋亡指数高的患者生存时间明显延长。骨肉瘤组织细胞凋亡指数与肿瘤大小和血清碱性磷酸酶水平相关，而与肿瘤的病理分级和临床分期无关。
本研究结果表明，骨肉瘤组织细胞凋亡指数联合血清碱性磷酸酶可作为预测骨肉瘤恶性程度和预后的有效指标，有助于提高骨肉瘤的临床治疗效果。
