de Oliveira Denise Hélen Imaculada Pereira, da Silveira Ericka Janine Dantas, de Medeiros Ana Miryam Costa, Alves Pollianna Muniz, Queiroz Lélia Maria Guedes
Postgraduate Program, Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
J Oral Pathol Med. 2014 Jan;43(1):76-80. doi: 10.1111/jop.12092. Epub 2013 Jun 4.
To investigate whether the immunohistochemical expression of GLUT-1 and HIF-1α is related to the diagnosis and pathogenesis of oral vascular lesions.
Thirty cases each of pyogenic granuloma (PG) and hemangioma were studied. Antibodies against GLUT-1 and HIF-1α were detected by immunoperoxidase staining in 3-μm histological sections, and the results were analyzed qualitatively and quantitatively, respectively. Positive and negative cells were counted, and the mean number of positive cells was calculated for each case.
The initial diagnosis of hemangioma was maintained in only 7 (23%) of the 30 cases studied, which were positive for GLUT-1. The remaining 23 cases were reclassified as vascular malformation (VM) (n = 13) and PG (n = 10) due to the absence of staining. The endothelium of blood vessels was negative for GLUT-1 in all cases initially diagnosed as PG (n = 30). The percentage of HIF-1α-positive cells was higher in cases of PG, followed by hemangiomas and VMs (P = 0.005).
Histological features are not sufficient to establish the correct diagnosis of oral hemangiomas, and an accurate anamnesis is essential in these cases. In addition, these findings demonstrate that vascular lesions express mediators of angiogenesis, HIF-1α, and suggest that his process may play a role in the pathogenesis of vascular.
研究葡萄糖转运蛋白1（GLUT-1）和缺氧诱导因子-1α（HIF-1α）的免疫组化表达与口腔血管性病变的诊断及发病机制是否相关。
对30例化脓性肉芽肿（PG）和30例血管瘤进行研究。采用免疫过氧化物酶染色法在3μm组织切片上检测抗GLUT-1和HIF-1α抗体，分别对结果进行定性和定量分析。计数阳性和阴性细胞，计算每例阳性细胞的平均数。
在研究的30例病例中，仅7例（23%）最初诊断为血管瘤的病例GLUT-1呈阳性。其余23例因无染色而重新分类为血管畸形（VM）（n = 13）和PG（n = 10）。所有最初诊断为PG的病例（n = 30）血管内皮GLUT-1均为阴性。PG病例中HIF-阳性细胞百分比更高，其次是血管瘤和VM（P = 0.005）。
组织学特征不足以准确诊断口腔血管瘤，在这些病例中准确的病史至关重要。此外，这些发现表明血管性病变表达血管生成介质HIF-1α，提示该过程可能在血管发病机制中起作用。
