Institute of Epidemiology and Microbiology, Siberian Branch of Russian Academy of Medical Sciences, Vladivostok, Russian Federation.
Virology. 2013 Aug 15;443(1):89-98. doi: 10.1016/j.virol.2013.04.029. Epub 2013 Jun 2.
We analyzed the genetics and virulence of 35 strains of TBEV isolated from patients with different forms of the infection living in the southern Far East region of Russia. The results of moleculargenetics studies of the TBEV strains showed that most of the strains that cause inapparent infections form a single cluster (I) with the Oshima 5-10 strain from Japan on the phylogenetic tree. A comparison of the amino acid sequences of the viral polyproteins of the studied strains identified 17 amino acid residues distributed unevenly across the polyprotein that distinctly differed between the clusters of inapparent and virulent strains. We detected additional substitutions in the NS1 and NS5 proteins. These substitutions might influence the pathogenic potential of the strains. Using a model of inbred mice of different ages, we examined the virulence of these strains and showed the different pathogenic potentials of strains belonging to different clusters.
我们分析了俄罗斯远东南部地区不同感染形式患者中分离出的 35 株 TBEV 的遗传学和毒力。TBEV 株的分子遗传学研究结果表明，引起无症状感染的大多数菌株在系统发育树上与来自日本的大岛 5-10 株形成一个单一的簇（I）。对研究株的病毒多蛋白的氨基酸序列比较鉴定出，在多蛋白上不均匀分布的 17 个氨基酸残基在无症状和毒力株的簇之间明显不同。我们在 NS1 和 NS5 蛋白中检测到额外的取代。这些取代可能会影响菌株的致病潜力。使用不同年龄的近交系小鼠模型，我们检查了这些菌株的毒力，并显示了属于不同簇的菌株的不同致病潜力。
